| pubmed-article:10544116 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10544116 | lifeskim:mentions | umls-concept:C0376526 | lld:lifeskim |
| pubmed-article:10544116 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:10544116 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10544116 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10544116 | pubmed:dateCreated | 1999-11-18 | lld:pubmed |
| pubmed-article:10544116 | pubmed:abstractText | The ORF50 gene of Kaposi's sarcoma (KS)-associated herpesvirus, or human herpesvirus 8 (KSHV), activates viral replication and is weakly homologous to the herpesvirus family of R transactivators; therefore, the transcription and translation events from this region of KSHV are key events in viral reactivation. We demonstrate that ORF50 is expressed in a bicistronic message after induction of the viral lytic cycle. ORF50 migrated as a series of polypeptides: the major ones as 119 and 101 kDa, respectively. Using 3' rapid amplification of cDNA ends, RT-PCR, and cDNA library screening, we demonstrate that the major ORF50 transcript also encodes K8. The ORF50/K8 transcript was resistant to cyclohexamide, whereas the K8 transcript was only partially resistant to cyclohexamide at early timepoints. Both transcripts showed partial resistance after 12 h of phorbol ester induction. Using a GAL4-ORF50 fusion protein expression vector, we demonstrate that the transactivation domain of ORF50 resides within a 160-amino-acid region of the carboxyl portion of the ORF. Upstream regions of both ORF50 and K8 have basal promoter activity in KSHV-infected cells. K8, which had sequence homology to Bzip proteins, did not activate either promoter. However, both promoters were activated after cotransfection of ORF50 in BCBL-1 cells. | lld:pubmed |
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| pubmed-article:10544116 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10544116 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10544116 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10544116 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:10544116 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:YuTT | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:QuinlivanE... | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:WangR XRX | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:SeamanW TWT | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:WeisseMM | lld:pubmed |
| pubmed-article:10544116 | pubmed:author | pubmed-author:HaleE EEE | lld:pubmed |
| pubmed-article:10544116 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10544116 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10544116 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:10544116 | pubmed:volume | 263 | lld:pubmed |
| pubmed-article:10544116 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10544116 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10544116 | pubmed:pagination | 436-49 | lld:pubmed |
| pubmed-article:10544116 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10544116 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10544116 | pubmed:articleTitle | Gene expression from the ORF50/K8 region of Kaposi's sarcoma-associated herpesvirus. | lld:pubmed |
| pubmed-article:10544116 | pubmed:affiliation | Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA. | lld:pubmed |
| pubmed-article:10544116 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10544116 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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