| pubmed-article:10526220 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0162713 | lld:lifeskim |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0311404 | lld:lifeskim |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10526220 | lifeskim:mentions | umls-concept:C0813983 | lld:lifeskim |
| pubmed-article:10526220 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10526220 | pubmed:dateCreated | 1999-11-2 | lld:pubmed |
| pubmed-article:10526220 | pubmed:abstractText | The oxidative DNA damage induced by the polar photosensitizer Ro19-8022 in the presence of light was studied and correlated with the associated mutagenicity. Both in isolated DNA and AS52 Chinese hamster ovary cells, photoexcited Ro19-8022 gave rise to a DNA damage profile that was similar to that caused by singlet oxygen: base modifications sensitive to the repair endonuclease Fpg protein, which according to high-performance liquid chromatography (HPLC) analysis were predominantly 8-hydroxyguanine (8-oxoG) residues, were generated in much higher yield than single-strand breaks, sites of base loss (AP sites) and oxidative pyrimidine modifications sensitive to endonuclease III. Fifty percent of the Fpg-sensitive modifications were repaired within 2 h. Under conditions that induced 10 Fpg-sensitive modifications per 10(6) bp (six 8-oxoG residues per 10(6) bp), approximately 60 mutations per 10(6) cells were induced in the gpt locus of the AS52 cells. A rather similar mutation frequency was observed when a plasmid carrying the gpt gene was exposed to Ro19-8022 plus light under cell-free conditions and subsequently replicated in bacteria. Sequence analysis revealed that GC-->TA and GC-->CG transversions accounted for 90% of the base substitutions. A significant generation of micronuclei was detectable in AS52 cells exposed to the photosensitizer plus light as well. | lld:pubmed |
| pubmed-article:10526220 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10526220 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10526220 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10526220 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10526220 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10526220 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10526220 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10526220 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10526220 | pubmed:issn | 0027-5107 | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:SchulzII | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:GockeEE | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:PflaumMM | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:WildWW | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:EckertII | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:EpeBB | lld:pubmed |
| pubmed-article:10526220 | pubmed:author | pubmed-author:MahlerH CHC | lld:pubmed |
| pubmed-article:10526220 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10526220 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:10526220 | pubmed:volume | 435 | lld:pubmed |
| pubmed-article:10526220 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10526220 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10526220 | pubmed:pagination | 89-101 | lld:pubmed |
| pubmed-article:10526220 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10526220 | pubmed:meshHeading | pubmed-meshheading:10526220... | lld:pubmed |
| pubmed-article:10526220 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10526220 | pubmed:articleTitle | Oxidative DNA damage and mutations induced by a polar photosensitizer, Ro19-8022. | lld:pubmed |
| pubmed-article:10526220 | pubmed:affiliation | Institute of Pharmacy, University of Mainz, Germany. | lld:pubmed |
| pubmed-article:10526220 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10526220 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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