| pubmed-article:10523756 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10523756 | lifeskim:mentions | umls-concept:C0382336 | lld:lifeskim |
| pubmed-article:10523756 | lifeskim:mentions | umls-concept:C1514716 | lld:lifeskim |
| pubmed-article:10523756 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:10523756 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10523756 | pubmed:dateCreated | 1999-12-10 | lld:pubmed |
| pubmed-article:10523756 | pubmed:abstractText | Western blots using an antibody which recognizes the orphanin FQ/nociceptin (OFQ/N) receptor reveals a band at approximately 69 kD in several cell lines, including the Raji human B cell lymphoma cell line. RT-PCR confirms the presence of this receptor in the Raji cells. Binding studies revealed a high affinity [(125)I][Tyr(14)]OFQ/N site in the Raji cells. The affinity of [(125)I][Tyr(14)]OFQ/N in the Raji cells (K(D) 68.4 pM) was similar to that in the transfected receptor (K(D) 36.7 pM). Its selectivity profile also was quite similar. OFQ/N competed binding quite potently (K(i) 65 pM), as did [Tyr(14)]OFQ/N (K(i) 33 pM). Traditional opioids displayed no appreciable affinity for the binding at any concentration examined, with the exception of naloxone benzoylhydrazone, which had only a very modest affinity. The receptors in the Raji cells were functionally active. OFQ/N inhibited forskolin-stimulated cyclase by 72% with an IC(50) value of approximately 1 nM. | lld:pubmed |
| pubmed-article:10523756 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10523756 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10523756 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10523756 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10523756 | pubmed:issn | 0887-4476 | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:GoldbergII | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:PasternakG... | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:MathisJJ | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:ScheinbergD... | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:PanY XYX | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:BrooksA IAI | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:Ryan-MoroJJ | lld:pubmed |
| pubmed-article:10523756 | pubmed:author | pubmed-author:LawM DMDJr | lld:pubmed |
| pubmed-article:10523756 | pubmed:copyrightInfo | Copyright 1999 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10523756 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10523756 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:10523756 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10523756 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10523756 | pubmed:pagination | 187-91 | lld:pubmed |
| pubmed-article:10523756 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
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| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
| pubmed-article:10523756 | pubmed:meshHeading | pubmed-meshheading:10523756... | lld:pubmed |
| pubmed-article:10523756 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10523756 | pubmed:articleTitle | [(125)I]orphanin FQ/nociceptin binding in Raji cells. | lld:pubmed |
| pubmed-article:10523756 | pubmed:affiliation | The Laboratory of Hematopoietic Cancer Immunochemistry and The Cotzias Laboratory of Neuro-Oncology, The Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA. | lld:pubmed |
| pubmed-article:10523756 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10523756 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
