| pubmed-article:10510675 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10510675 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:10510675 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:10510675 | lifeskim:mentions | umls-concept:C1515705 | lld:lifeskim |
| pubmed-article:10510675 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:10510675 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10510675 | pubmed:dateCreated | 1999-10-19 | lld:pubmed |
| pubmed-article:10510675 | pubmed:abstractText | We describe the usefulness of a real-time polymerase chain reaction (PCR) assay for detection of the t(11;14)(q13;q32), most commonly present in mantle cell lymphoma (MCL). This assay is based on the 5'-->3' exonuclease activity of Taq polymerase, which cleaves an internal probe labeled with a reporter dye at its 5' end and a quencher dye at its 3' end during PCR. The real-time t(11;14) PCR assay was established using DNA from a case of MCL with the t(11;14), amplifiable using conventional PCR and primers specific for the major translocation cluster (MTC) region of the bcl-1 locus and the immunoglobulin heavy chain joining region gene (JH). The specificity was determined by analyzing DNA from 82 cases: 50 MCL, 27 other types of non-Hodgkin lymphoma (NHL), and 5 reactive lymphoid proliferations. The real-time t(11;14) PCR results were correlated with data obtained by a conventional PCR assay. By using the real-time assay, bcl-1 MTC/JH DNA fusion sequences were detected in 25 of 50 MCLs but not in other NHLs or reactive lymphoid proliferations. Concordance between real-time and conventional PCR methods for MCL was 96% and for all samples was 98%. The results demonstrate that this real-time PCR method to detect bcl-1 MTC/JH DNA fusion sequences is specific and reliable. In addition, the results are available immediately following amplification, without standard post-PCR manipulations. | lld:pubmed |
| pubmed-article:10510675 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10510675 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10510675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10510675 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10510675 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:10510675 | pubmed:issn | 0002-9173 | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:SarrisA HAH | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:LuthraRR | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:MedeirosL JLJ | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:HanVV | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:RomagueraJ... | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:CabanillasF... | lld:pubmed |
| pubmed-article:10510675 | pubmed:author | pubmed-author:PaladuguA VAV | lld:pubmed |
| pubmed-article:10510675 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10510675 | pubmed:volume | 112 | lld:pubmed |
| pubmed-article:10510675 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10510675 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10510675 | pubmed:pagination | 524-30 | lld:pubmed |
| pubmed-article:10510675 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:10510675 | pubmed:meshHeading | pubmed-meshheading:10510675... | lld:pubmed |
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| pubmed-article:10510675 | pubmed:meshHeading | pubmed-meshheading:10510675... | lld:pubmed |
| pubmed-article:10510675 | pubmed:meshHeading | pubmed-meshheading:10510675... | lld:pubmed |
| pubmed-article:10510675 | pubmed:meshHeading | pubmed-meshheading:10510675... | lld:pubmed |
| pubmed-article:10510675 | pubmed:meshHeading | pubmed-meshheading:10510675... | lld:pubmed |
| pubmed-article:10510675 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10510675 | pubmed:articleTitle | Real-time 5'-->3' exonuclease-based PCR assay for detection of the t(11;14)(q13;q32). | lld:pubmed |
| pubmed-article:10510675 | pubmed:affiliation | Department of Pathology, University of Texas MD, Anderson Cancer Center, Houston 77030, USA. | lld:pubmed |
| pubmed-article:10510675 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10510675 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10510675 | lld:pubmed |
