| pubmed-article:10490615 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0206131 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0032214 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0600138 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C0178666 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C1554080 | lld:lifeskim |
| pubmed-article:10490615 | lifeskim:mentions | umls-concept:C1706198 | lld:lifeskim |
| pubmed-article:10490615 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:10490615 | pubmed:dateCreated | 2000-2-3 | lld:pubmed |
| pubmed-article:10490615 | pubmed:abstractText | We evaluated the role of the G alpha-q (Galphaq) subunit of heterotrimeric G proteins in the insulin signaling pathway leading to GLUT4 translocation. We inhibited endogenous Galphaq function by single cell microinjection of anti-Galphaq/11 antibody or RGS2 protein (a GAP protein for Galphaq), followed by immunostaining to assess GLUT4 translocation in 3T3-L1 adipocytes. Galphaq/11 antibody and RGS2 inhibited insulin-induced GLUT4 translocation by 60 or 75%, respectively, indicating that activated Galphaq is important for insulin-induced glucose transport. We then assessed the effect of overexpressing wild-type Galphaq (WT-Galphaq) or a constitutively active Galphaq mutant (Q209L-Galphaq) by using an adenovirus expression vector. In the basal state, Q209L-Galphaq expression stimulated 2-deoxy-D-glucose uptake and GLUT4 translocation to 70% of the maximal insulin effect. This effect of Q209L-Galphaq was inhibited by wortmannin, suggesting that it is phosphatidylinositol 3-kinase (PI3-kinase) dependent. We further show that Q209L-Galphaq stimulates PI3-kinase activity in p110alpha and p110gamma immunoprecipitates by 3- and 8-fold, respectively, whereas insulin stimulates this activity mostly in p110alpha by 10-fold. Nevertheless, only microinjection of anti-p110alpha (and not p110gamma) antibody inhibited both insulin- and Q209L-Galphaq-induced GLUT4 translocation, suggesting that the metabolic effects induced by Q209L-Galphaq are dependent on the p110alpha subunit of PI3-kinase. In summary, (i) Galphaq appears to play a necessary role in insulin-stimulated glucose transport, (ii) Galphaq action in the insulin signaling pathway is upstream of and dependent upon PI3-kinase, and (iii) Galphaq can transmit signals from the insulin receptor to the p110alpha subunit of PI3-kinase, which leads to GLUT4 translocation. | lld:pubmed |
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| pubmed-article:10490615 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10490615 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10490615 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10490615 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10490615 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:10490615 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:EgawaKK | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:BrownJ HJH | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:ImamuraTT | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:VIGPP | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:IshibashiKK | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:OlefskyJ MJM | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:AdamsJ WJW | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:NakashimaNN | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:ClodeAA | lld:pubmed |
| pubmed-article:10490615 | pubmed:author | pubmed-author:VollenweiderP... | lld:pubmed |
| pubmed-article:10490615 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10490615 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:10490615 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10490615 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10490615 | pubmed:pagination | 6765-74 | lld:pubmed |
| pubmed-article:10490615 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10490615 | pubmed:meshHeading | pubmed-meshheading:10490615... | lld:pubmed |
| pubmed-article:10490615 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10490615 | pubmed:articleTitle | G alpha-q/11 protein plays a key role in insulin-induced glucose transport in 3T3-L1 adipocytes. | lld:pubmed |
