Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:10454998rdf:typepubmed:Citationlld:pubmed
pubmed-article:10454998lifeskim:mentionsumls-concept:C0043393lld:lifeskim
pubmed-article:10454998lifeskim:mentionsumls-concept:C0598312lld:lifeskim
pubmed-article:10454998lifeskim:mentionsumls-concept:C1533691lld:lifeskim
pubmed-article:10454998lifeskim:mentionsumls-concept:C1518434lld:lifeskim
pubmed-article:10454998pubmed:issue3lld:pubmed
pubmed-article:10454998pubmed:dateCreated1999-9-16lld:pubmed
pubmed-article:10454998pubmed:abstractTextTwo assays have been developed for studying DNA replication in vitro based on nuclear extracts isolated from budding yeast cells synchronized in S phase. In the first, the template DNA for replication is provided in the form of intact yeast nuclei, usually from cells arrested in G(1). In the second assay, bacterially produced supercoiled plasmid is replicated in an S-phase nuclear extract supplemented with nucleotides and an energy-regenerating system. Semiconservative DNA replication is monitored by substitution of newly synthesized DNA with bromodeoxyuridine 5'-triphosphate (BrdUTP) and density gradient analysis. In addition, neutral-neutral two-dimensional gel analyses and, in the case of nuclei, detection of newly synthesized DNA in replication foci by DIG-dUTP incorporation can be used to monitor replication.lld:pubmed
pubmed-article:10454998pubmed:languageenglld:pubmed
pubmed-article:10454998pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10454998pubmed:citationSubsetIMlld:pubmed
pubmed-article:10454998pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10454998pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10454998pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10454998pubmed:statusMEDLINElld:pubmed
pubmed-article:10454998pubmed:monthJullld:pubmed
pubmed-article:10454998pubmed:issn1046-2023lld:pubmed
pubmed-article:10454998pubmed:authorpubmed-author:GasserS MSMlld:pubmed
pubmed-article:10454998pubmed:authorpubmed-author:PaseroPPlld:pubmed
pubmed-article:10454998pubmed:authorpubmed-author:DunckerB PBPlld:pubmed
pubmed-article:10454998pubmed:copyrightInfoCopyright 1999 Academic Press.lld:pubmed
pubmed-article:10454998pubmed:issnTypePrintlld:pubmed
pubmed-article:10454998pubmed:volume18lld:pubmed
pubmed-article:10454998pubmed:ownerNLMlld:pubmed
pubmed-article:10454998pubmed:authorsCompleteYlld:pubmed
pubmed-article:10454998pubmed:pagination368-76, 323lld:pubmed
pubmed-article:10454998pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:meshHeadingpubmed-meshheading:10454998...lld:pubmed
pubmed-article:10454998pubmed:year1999lld:pubmed
pubmed-article:10454998pubmed:articleTitleIn vitro DNA replication in yeast nuclear extracts.lld:pubmed
pubmed-article:10454998pubmed:affiliationSwiss Institute for Experimental Cancer Research (ISREC), Ch. des Boveresses 155, Epalinges/Lausanne, CH-1066, Switzerland.lld:pubmed
pubmed-article:10454998pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10454998pubmed:publicationTypeIn Vitrolld:pubmed
pubmed-article:10454998pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:10454998lld:pubmed