| pubmed-article:10405338 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0004112 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0218584 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0243102 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C2745878 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:10405338 | lifeskim:mentions | umls-concept:C0443264 | lld:lifeskim |
| pubmed-article:10405338 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10405338 | pubmed:dateCreated | 1999-8-9 | lld:pubmed |
| pubmed-article:10405338 | pubmed:abstractText | Astrocytes express endothelin-1 (ET-1), ET-3, and their receptors, ET(A) and ET(B). We report here that activated astrocytes in vivo also express endothelin converting enzyme-1 (ECE-1). Higher basal ET-1 concentrations in astrocyte media from ET(B)-deficient (sl/sl) versus wildtype (+/+) rats suggested that altered ECE activity may be related to the absence of ET(B) receptors. Quantification of ECE activity in membranes from sl/sl astrocytes yielded a 50% higher conversion compared to +/+ astrocytes, with indistinguishable ECE-1 mRNA and protein levels. Kinetic analysis of ECE activity revealed similar V(max) values in sl/sl and +/+ astrocytes. Enzyme activity was competitively inhibited by phosphoramidon with K(i) values of 0. 6 and 0.3 microM, respectively. The K(m) value of ECE was 0.5 microM in +/+ and 0.2 microM in sl/sl astrocytes. Two-dimensional focussing of astrocytic ECE-1 uncovered heterogeneity of charge and molecular weight. ECE-1 from sl/sl revealed a glycosylation pattern different from +/+ astrocytes. In conclusion, the ET(B) receptor may, via ECE-1 glycosylation, exert a negative feedback on ECE activity in the astrocytic endothelin system. | lld:pubmed |
| pubmed-article:10405338 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10405338 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10405338 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10405338 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10405338 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:SirénA LAL | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:LangerDD | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:OldenburgJJ | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:EhrenreichHH | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:LöfflerB MBM | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:SchillingLL | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:SubkowskiTT | lld:pubmed |
| pubmed-article:10405338 | pubmed:author | pubmed-author:HasselblattMM | lld:pubmed |
| pubmed-article:10405338 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10405338 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10405338 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:10405338 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:10405338 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10405338 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10405338 | pubmed:pagination | 149-55 | lld:pubmed |
| pubmed-article:10405338 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10405338 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10405338 | pubmed:articleTitle | Endothelin converting enzyme activity in primary rat astrocytes is modulated by endothelin B receptors. | lld:pubmed |
| pubmed-article:10405338 | pubmed:affiliation | Departments of Neurology, Georg-August-University, Göttingen, Germany. ehrenreich@exmax1.mpiem.gwdg.de | lld:pubmed |
| pubmed-article:10405338 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10405338 | lld:pubmed |
