| pubmed-article:10385523 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C0042219 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C0332466 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C0205224 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C1506158 | lld:lifeskim |
| pubmed-article:10385523 | lifeskim:mentions | umls-concept:C1506159 | lld:lifeskim |
| pubmed-article:10385523 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:10385523 | pubmed:dateCreated | 1999-8-2 | lld:pubmed |
| pubmed-article:10385523 | pubmed:abstractText | Vacuole SNAREs, including the t-SNAREs Vam3p and Vam7p and the v-SNARE Nyv1p, are found in a multisubunit "cis" complex on isolated organelles. We now identify the v-SNAREs Vti1p and Ykt6p by mass spectrometry as additional components of the immunoisolated vacuolar SNARE complex. Immunodepletion of detergent extracts with anti-Vti1p removes all the Ykt6p that is in a complex with Vam3p, immunodepletion with anti-Ykt6p removes all the Vti1p that is complexed with Vam3p, and immunodepletion with anti-Nyv1p removes all the Ykt6p in complex with other SNAREs, demonstrating that they are all together in the same cis multi-SNARE complex. After priming, which disassembles the cis-SNARE complex, antibodies to any of the five SNARE proteins still inhibit the fusion assay until the docking stage is completed, suggesting that each SNARE plays a role in docking. Furthermore, vti1 temperature-sensitive alleles cause a synthetic fusion-defective phenotype in our reaction. Our data show that vacuole-vacuole fusion requires a cis-SNARE complex of five SNAREs, the t-SNAREs Vam3p and Vam7p and the v-SNAREs Nyv1p, Vti1p, and Ykt6p. | lld:pubmed |
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| pubmed-article:10385523 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10385523 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10385523 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10385523 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10385523 | pubmed:issn | 0021-9525 | lld:pubmed |
| pubmed-article:10385523 | pubmed:author | pubmed-author:WicknerWW | lld:pubmed |
| pubmed-article:10385523 | pubmed:author | pubmed-author:StevensT HTH | lld:pubmed |
| pubmed-article:10385523 | pubmed:author | pubmed-author:JensenO NON | lld:pubmed |
| pubmed-article:10385523 | pubmed:author | pubmed-author:MargolisNN | lld:pubmed |
| pubmed-article:10385523 | pubmed:author | pubmed-author:von... | lld:pubmed |
