| pubmed-article:10358066 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0016956 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C1412716 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10358066 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10358066 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:10358066 | pubmed:dateCreated | 1999-7-6 | lld:pubmed |
| pubmed-article:10358066 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:abstractText | We isolated a cDNA encoding a novel glucuronyltransferase, designated GlcAT-D, involved in the biosynthesis of the HNK-1 carbohydrate epitope from rat embryo cDNA by the degenerate polymerase chain reaction method. The new cDNA sequence revealed an open reading frame coding for a protein of 324 amino acids with type II transmembrane protein topology. The amino acid sequence of GlcAT-D displayed 50.0% identity to rat GlcAT-P, which is involved in the biosynthesis of the HNK-1 epitope on glycoproteins. Expression of GlcAT-D in COS-7 cells resulted in the formation of the HNK-1 epitope on the cell surface. The enzyme expressed in COS-7 cells transferred a glucuronic acid (GlcA) not only to asialo-orosomucoid, a glycoprotein bearing terminal N-acetyllactosamine structure, but also to paragloboside (lacto-N-neotetraosylceramide), a precursor of the HNK-1 epitope on glycolipids. Furthermore, substrate specificity analysis using a soluble chimeric form of GlcAT-D revealed that GlcAT-D transfers a GlcA not only to Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine++ + but also to Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-pyridylamine++ +. Enzymatic hydrolysis and Smith degradation of the reaction product indicated that GlcAT-D transfers a GlcA through a beta1,3-linkage to a terminal galactose. The GlcAT-D transcripts were detected in embryonic, postnatal, and adult rat brain. In situ hybridization analysis revealed that the expression pattern of GlcAT-D transcript in embryo is similar to that of GlcAT-P, but distinct expression of GlcAT-D was observed in the embryonic pallidum and retina. Regions that expressed GlcAT-D and/or GlcAT-P were always HNK-1-positive, indicating that both GlcATs are involved in the synthesis of the HNK-1 epitope in vivo. | lld:pubmed |
| pubmed-article:10358066 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10358066 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10358066 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10358066 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10358066 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:ShimodaYY | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:SanaiYY | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:HarioMM | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:TajimaYY | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:NagaseTT | lld:pubmed |
| pubmed-article:10358066 | pubmed:author | pubmed-author:OsumiNN | lld:pubmed |
| pubmed-article:10358066 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10358066 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:10358066 | pubmed:volume | 274 | lld:pubmed |
| pubmed-article:10358066 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10358066 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10358066 | pubmed:pagination | 17115-22 | lld:pubmed |
| pubmed-article:10358066 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10358066 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10358066 | pubmed:articleTitle | Cloning and expression of a novel galactoside beta1, 3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope. | lld:pubmed |
| pubmed-article:10358066 | pubmed:affiliation | Department of Biochemical Cell Research, Tokyo Metropolitan Institute of Medical Science (RINSHOKEN), Tokyo 113-8613, Japan. | lld:pubmed |
| pubmed-article:10358066 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10358066 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10358066 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:64544 | entrezgene:pubmed | pubmed-article:10358066 | lld:entrezgene |
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