| pubmed-article:10348918 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C0034963 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C0596260 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C1554963 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C0052898 | lld:lifeskim |
| pubmed-article:10348918 | lifeskim:mentions | umls-concept:C0596335 | lld:lifeskim |
| pubmed-article:10348918 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10348918 | pubmed:dateCreated | 1999-11-8 | lld:pubmed |
| pubmed-article:10348918 | pubmed:abstractText | Carboxyl group modification with DCCD and NCD-4 was employed to investigate the chemical environment of the side chains of archaeopsin-1 (aO-1) and bacterioopsin (bO). Some differences were observed between aO-1 and bO. Although DCCD or NCD-4 did not modify aO-1 in bleached membrane, they modified bO in bleached membrane and in mixed DMPC/CHAPS/SDS micelles at neutral pH, thereby affecting the opsin shift and the photocycle of the regenerated chromophore. On the contrary, after solubilization with SDS, aO-1 and bO were modified by DCCD and NCD-4, which decreased the chromophore regeneration. In particular, the reaction of aO-1 in SDS with NCD-4 proceeded in a 1:1 ratio at neutral pH. The fluorescence and CD spectra indicated that the modified site was located in the hydrophobic, asymmetrical region. Lysyl-endopeptidase digestion of NCD-4 modified aO-1 produced a fluorescent fragment and amino acid sequence analysis showed that Asp85 or Asp96 in helix C is a probable candidate for the modified residue at present. Kinetic CD measurements revealed that the introduction of N-acylurea at an Asp residue in helix C did not affect the formation of the transient intermediate but inhibited the side chain packing during refolding. | lld:pubmed |
| pubmed-article:10348918 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10348918 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10348918 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10348918 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10348918 | pubmed:issn | 0021-924X | lld:pubmed |
| pubmed-article:10348918 | pubmed:author | pubmed-author:FujiiKK | lld:pubmed |
| pubmed-article:10348918 | pubmed:author | pubmed-author:MukohataYY | lld:pubmed |
| pubmed-article:10348918 | pubmed:author | pubmed-author:SugiyamaYY | lld:pubmed |
| pubmed-article:10348918 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10348918 | pubmed:volume | 125 | lld:pubmed |
| pubmed-article:10348918 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10348918 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10348918 | pubmed:pagination | 1144-50 | lld:pubmed |
| pubmed-article:10348918 | pubmed:dateRevised | 2007-12-19 | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:meshHeading | pubmed-meshheading:10348918... | lld:pubmed |
| pubmed-article:10348918 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10348918 | pubmed:articleTitle | The effect of carboxyl group modification on the chromophore regeneration of archaeopsin-1 and bacterioopsin. | lld:pubmed |
| pubmed-article:10348918 | pubmed:affiliation | Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan. ysugiya@bio.nagoya-u.ac.jp | lld:pubmed |
| pubmed-article:10348918 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10348918 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
