Linked Life Data

10338299

Source:http://linkedlifedata.com/resource/pubmed/id/10338299

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pubmed-article:10338299pubmed:abstractTextThe actions of serotonin were investigated on motoneurons isolated from embryonic day 14 rat spinal cord and enriched by metrizamide density gradient centrifugation. Trophic support was provided by a spinal cord glial monolayer, ciliary neurotrophic factor and heat-inactivated serum. Cultures were maintained for 17-83 days and investigated using whole-cell patch-clamp recording. Serotonin evoked slow depolarizations (6.2+/-0.7 or 9.3+/-1.3 mV in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione and strychnine, EC50 8.2 nM), which were reversibly blocked by 0.1 microM ketanserin. Serotonin generated synaptic potentials in motoneurons, lowered the threshold for repetitive firing and changed the slope of the current intensity-firing frequency relationship. The inward current evoked by serotonin (-147+/-15.2 pA) was ascribed to a complex ionic mechanism, which varied amongst neurons in the sampled population. It was due to closure of barium-sensitive potassium channels, effects on Ih and increase in a separate mixed cation current which comprised both transient voltage-sensitive and sustained components. We conclude that serotonergic responses develop in motoneurons cultured under these conditions in the absence of serotonergic input, sensory neurons or many interneurons.lld:pubmed
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pubmed-article:10338299pubmed:dateRevised2009-9-29lld:pubmed
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pubmed-article:10338299pubmed:articleTitleIonic mechanisms underlying excitatory effects of serotonin on embryonic rat motoneurons in long-term culture.lld:pubmed
pubmed-article:10338299pubmed:affiliationPhysiology Unit, School of Molecular and Medical Biosciences, Cardiff University, UK.lld:pubmed
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pubmed-article:10338299pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:10338299pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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