| pubmed-article:10191039 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0205252 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0015915 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0020676 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0015075 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C0037633 | lld:lifeskim |
| pubmed-article:10191039 | lifeskim:mentions | umls-concept:C2936610 | lld:lifeskim |
| pubmed-article:10191039 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10191039 | pubmed:dateCreated | 1999-6-9 | lld:pubmed |
| pubmed-article:10191039 | pubmed:abstractText | Two-cell embryos derived from immature rdw rats by in vitro fertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viability in vitro. All embryos exposed to EFS20 for 1-3 min before vitrification in EFS40 were morphologically normal. However, 2-3 min of exposure to EFS20 increased the number of embryos that developed beyond the four-cell stage. More embryos exposed to EFS20 for 2-3 min developed to morulae (63-64%) and blastocysts (34-38%) than those exposed for 1 min (35 and 10%, respectively). After transfer, 33% of embryos exposed to EFS20 for 3 min and vitrified in EFS40 developed to term compared to 29% of fresh embryos. Fifteen (47%) of live young were homozygous rdw and all of the others were heterozygous rats. The present study demonstrated that vitrification in EFS solution can be routinely used to cryopreserve rat two-cell IVF-embryos with no loss of viability. | lld:pubmed |
| pubmed-article:10191039 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10191039 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10191039 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10191039 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10191039 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10191039 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10191039 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10191039 | pubmed:issn | 0011-2240 | lld:pubmed |
| pubmed-article:10191039 | pubmed:author | pubmed-author:SatoEE | lld:pubmed |
| pubmed-article:10191039 | pubmed:author | pubmed-author:UmezuMM | lld:pubmed |
| pubmed-article:10191039 | pubmed:author | pubmed-author:JiangJ YJY | lld:pubmed |
| pubmed-article:10191039 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10191039 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10191039 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:10191039 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10191039 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10191039 | pubmed:pagination | 160-4 | lld:pubmed |
| pubmed-article:10191039 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:meshHeading | pubmed-meshheading:10191039... | lld:pubmed |
| pubmed-article:10191039 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10191039 | pubmed:articleTitle | Vitrification of two-cell rat embryos derived from immature hypothyroid rdw rats by in vitro fertilization in ethylene glycol-based solutions. | lld:pubmed |
| pubmed-article:10191039 | pubmed:affiliation | Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan. jiang@bios.tohoku.ac.jp | lld:pubmed |
| pubmed-article:10191039 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10191039 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
