| pubmed-article:10085297 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10085297 | lifeskim:mentions | umls-concept:C0887870 | lld:lifeskim |
| pubmed-article:10085297 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:10085297 | lifeskim:mentions | umls-concept:C0767546 | lld:lifeskim |
| pubmed-article:10085297 | lifeskim:mentions | umls-concept:C1422760 | lld:lifeskim |
| pubmed-article:10085297 | lifeskim:mentions | umls-concept:C0392752 | lld:lifeskim |
| pubmed-article:10085297 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10085297 | pubmed:dateCreated | 1999-6-10 | lld:pubmed |
| pubmed-article:10085297 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10085297 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10085297 | pubmed:abstractText | We recently isolated a novel actin filament (F-actin)-binding protein, afadin, that has two isoforms, l- and s-afadins. l-Afadin is ubiquitously expressed and specifically localized at zonula adherens (ZA) in epithelial cells and at cell-cell adherens junction (AJ) in nonepithelial cells, whereas s-afadin is abundantly expressed in neural tissue. l-Afadin has one PDZ domain, three proline-rich regions, and one F-actin-binding domain, whereas s-afadin lacks the third proline-rich region and the F-actin-binding domain. To understand the molecular mechanism of the specific localization of l-afadin at ZA in epithelial cells and at cell-cell AJ in nonepithelial cells, we attempted here to identify an l-afadin-binding protein(s) and isolated a protein, named ponsin. Ponsin had many splicing variants and the primary structures of two of them were determined. Both the two variants had three Src homology 3 (SH3) domains and turned out to be splicing variants of SH3P12. The third proline-rich region of l-afadin bound to the region of ponsin containing the second and third SH3 domains. Ponsin was ubiquitously expressed and localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells. Ponsin furthermore directly bound vinculin, an F-actin-binding protein localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells. Vinculin has one proline-rich region where two proline-rich sequences are located. The proline-rich region bound to the region of ponsin containing the first and second SH3 domains. l-Afadin and vinculin bound to ponsin in a competitive manner and these three proteins hardly formed a ternary complex. These results indicate that ponsin is an l-afadin- and vinculin-binding protein localized at ZA in epithelial cells, at cell-cell AJ in nonepithelial cells, and at cell-matrix AJ in both types of cells. | lld:pubmed |
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| pubmed-article:10085297 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10085297 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10085297 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10085297 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10085297 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10085297 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10085297 | pubmed:issn | 0021-9525 | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:TakahashiKK | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:TakaiYY | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:NakanishiHH | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:SatohKK | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:SatohAA | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:NishiokaHH | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:MizoguchiAA | lld:pubmed |
| pubmed-article:10085297 | pubmed:author | pubmed-author:MandaiKK | lld:pubmed |
| pubmed-article:10085297 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10085297 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:10085297 | pubmed:volume | 144 | lld:pubmed |
| pubmed-article:10085297 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10085297 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10085297 | pubmed:pagination | 1001-17 | lld:pubmed |
