Linked Life Data

10070092

Source:http://linkedlifedata.com/resource/pubmed/id/10070092

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pubmed-article:10070092pubmed:abstractTextThe purpose of this study was to test the hypothesis that increased flow through coronary arterioles increases endothelial cell nitric oxide synthase (ecNOS) and Cu/Zn superoxide dismutase (SOD) mRNA expression. Single porcine coronary arterioles (ID 100-160 micrometers; pressurized) were cannulated, perfused, and exposed to intraluminal flow sufficient to produce maximal flow-induced dilation of coronary arterioles (high flow; 7.52 +/- 0.22 microliter/min), low flow (0.84 +/- 0.05 microliter/min), or no flow for 2 or 4 h. Mean shear stress was calculated to be 5.7 +/- 1.0 dyn/cm2 for high-flow arterioles and 1. 6 +/- 1.0 dyn/cm2 for low-flow arterioles. At the end of the treatment period, mRNA was isolated from each vessel, and ecNOS and SOD mRNA expression was assessed using a semiquantitative RT-PCR. All data were standardized by coamplifying ecNOS or SOD with glyceraldehyde-3-phosphate dehydrogenase. The results indicate that ecNOS mRNA expression is increased in arterioles exposed to 2 or 4 h of high flow. In contrast, SOD mRNA expression was increased only after 4 h of high flow. Neither gene is induced by exposure to low flow. On the basis of these data, we concluded that ecNOS and SOD mRNA expression is regulated by flow in porcine coronary arterioles. In addition, we concluded that a threshold level of flow and shear stress must be sustained to elicit the upregulation of ecNOS and SOD mRNA expression.lld:pubmed
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pubmed-article:10070092pubmed:authorpubmed-author:MullerJ MJMlld:pubmed
pubmed-article:10070092pubmed:authorpubmed-author:LaughlinM HMHlld:pubmed
pubmed-article:10070092pubmed:authorpubmed-author:PriceE MEMlld:pubmed
pubmed-article:10070092pubmed:authorpubmed-author:WoodmanC RCRlld:pubmed
pubmed-article:10070092pubmed:authorpubmed-author:RushJ WJWlld:pubmed
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pubmed-article:10070092pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:10070092pubmed:articleTitleFlow regulation of ecNOS and Cu/Zn SOD mRNA expression in porcine coronary arterioles.lld:pubmed
pubmed-article:10070092pubmed:affiliationDepartment of Veterinary Biomedical Sciences and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211, USA. woodmanc@missouri.edulld:pubmed
pubmed-article:10070092pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10070092pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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