| pubmed-article:9951522 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C0007413 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C0031428 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C1522240 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C1407029 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:9951522 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:9951522 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9951522 | pubmed:dateCreated | 1999-3-1 | lld:pubmed |
| pubmed-article:9951522 | pubmed:abstractText | We describe the biocatalytic production of 3-phenylcatechol from 2-phenylphenol with the whole cell biocatalyst Escherichia coli JM101 (pHBP461). The recombinant produces 2-hydroxybiphenyl 3-monooxygenase, an enzyme from Pseudomonas azelaica HBP1. This enzyme introduces a hydroxyl-group at the C3-position of a variety of 2-substituted phenols, such as 2-phenylphenol. This permits the biocatalytic production of 3-substituted catechols, which are difficult to synthesize chemically. Both 2-phenylphenol and 3-phenylcatechol are highly toxic to E. coli. The toxic effects of 2-phenylphenol were minimized by feeding this substrate to the reactor at a rate slightly below the maximum biooxidation rate. As a result, the substrate concentration in the reactor remained below toxic levels during the bioconversion. The toxic product formed was removed by continuous adsorption on the solid resin Amberlite XAD-4. To this end the reaction mixture, containing the biocatalyst, was pumped continuously through an external loop with a fluidized bed of the resin. This resin efficiently and quantitatively adsorbed both 3-phenylcatechol and the remaining trace amounts of 2-phenylphenol. Consequently, the concentrations of these compounds were kept at subtoxic levels (below 100 mg L-1) and gram amounts of 3-phenylcatechol were produced with space-time yields of up to 0.39 g L-1 h-1. The product was recovered from the resin by acidic methanol elution and purified by recrystallization from n-hexane resulting in overall yields exceeding 59%. The optimized system served as a surprisingly simple and efficient integrated process, that allows the bioconversion of toxic substrates to toxic products with whole cell biocatalysts. | lld:pubmed |
| pubmed-article:9951522 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9951522 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9951522 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9951522 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9951522 | pubmed:issn | 0006-3592 | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:SchmidAA | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:HelmFF | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:WitholtBB | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:SuskoII | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:KohlerH PHP | lld:pubmed |
| pubmed-article:9951522 | pubmed:author | pubmed-author:WubboltsM GMG | lld:pubmed |
| pubmed-article:9951522 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9951522 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:9951522 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:9951522 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9951522 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9951522 | pubmed:pagination | 641-8 | lld:pubmed |
| pubmed-article:9951522 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
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| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
| pubmed-article:9951522 | pubmed:meshHeading | pubmed-meshheading:9951522-... | lld:pubmed |
| pubmed-article:9951522 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9951522 | pubmed:articleTitle | An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst. | lld:pubmed |
| pubmed-article:9951522 | pubmed:affiliation | Institute of Biotechnology, Swiss Federal Institute of Technology, Zürich, Switzerland. | lld:pubmed |
| pubmed-article:9951522 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9951522 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
