| pubmed-article:9950938 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9950938 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:9950938 | lifeskim:mentions | umls-concept:C0014609 | lld:lifeskim |
| pubmed-article:9950938 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:9950938 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:9950938 | lifeskim:mentions | umls-concept:C0010284 | lld:lifeskim |
| pubmed-article:9950938 | pubmed:issue | 2 Pt 2 | lld:pubmed |
| pubmed-article:9950938 | pubmed:dateCreated | 1999-3-30 | lld:pubmed |
| pubmed-article:9950938 | pubmed:abstractText | ATP-dependent Ca2+ uptake was determined into inside-out basolateral membrane vesicles (BLMV) from intermolt crayfish (Procambarus clarkii) Ca2+-transporting epithelia: gill, hepatopancreas (liver), and antennal gland (kidney). Extravesicular (EV) ATP (5 mM) increased 45Ca2+ uptake (free Ca2+ 5 microM) by fivefold but was abolished by pretreatment with either vanadate or the ionophore A-23187. Addition of A-23187 to Ca2+-loaded vesicles produced 70% efflux. The saturable carrier exhibited a Km for Ca2+ of 0.11-0.27 microM and maximal influx of 20-123 pmol. mg-1. min-1. The Km for ATP was 0.01-0.04 mM. The temperature coefficient ranged from 1.43 to 2.06. EGTA treatment of hepatopancreas and antennal gland vesicles decreased 45Ca2+ uptake by 50-90%; uptake was restorable by calmodulin. However, in gill, 45Ca2+ uptake was unaffected by EGTA treatment and calmodulin decreased uptake in both EGTA-treated and untreated vesicles. Addition of EV Na+ (5 mM) increased ATP-dependent Ca2+ uptake into hepatopancreas and antennal gland BLMV by 60%; in hepatopancreas BLMV, this increase was inhibitable by ouabain. However, ATP-dependent Ca2+ uptake in gill vesicles was Na+ independent. The relative role of each epithelium in whole animal Ca2+ homeostasis has been interpreted based on in vitro characteristics. | lld:pubmed |
| pubmed-article:9950938 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9950938 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9950938 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9950938 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9950938 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:9950938 | pubmed:author | pubmed-author:WeirJ PJP | lld:pubmed |
| pubmed-article:9950938 | pubmed:author | pubmed-author:WheatlyM GMG | lld:pubmed |
| pubmed-article:9950938 | pubmed:author | pubmed-author:PenceR CRC | lld:pubmed |
| pubmed-article:9950938 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9950938 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:9950938 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9950938 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9950938 | pubmed:pagination | R566-74 | lld:pubmed |
| pubmed-article:9950938 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:meshHeading | pubmed-meshheading:9950938-... | lld:pubmed |
| pubmed-article:9950938 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9950938 | pubmed:articleTitle | ATP-dependent calcium uptake into basolateral vesicles from transporting epithelia of intermolt crayfish. | lld:pubmed |
| pubmed-article:9950938 | pubmed:affiliation | Department of Biological Sciences, Wright State University, Dayton, Ohio 45435, USA. | lld:pubmed |
| pubmed-article:9950938 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9950938 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:9950938 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
