Linked Life Data

9930677

Source:http://linkedlifedata.com/resource/pubmed/id/9930677

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:9930677rdf:typepubmed:Citationlld:pubmed
pubmed-article:9930677lifeskim:mentionsumls-concept:C0205147lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0021034lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0456387lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C1709915lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0185026lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C1546857lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C2349182lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0205344lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0439828lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C1556066lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C1619636lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C1514873lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C2347858lld:lifeskim
pubmed-article:9930677lifeskim:mentionsumls-concept:C0719517lld:lifeskim
pubmed-article:9930677pubmed:issue12lld:pubmed
pubmed-article:9930677pubmed:dateCreated1999-3-23lld:pubmed
pubmed-article:9930677pubmed:abstractTextWhile studying the expression of single-chain antibodies (scFv) derived from several murine monoclonal antibodies, we found that residue 6 in Framework region 1 of the heavy chain variable domain plays a crucial role in antibody folding. Binding activity of three murine antibodies with a heavy chain variable region (VH) subgroup IIA was completely lost when at this position the wild-type residue glutamine (Q) was substituted by glutamate (E). Increased sensitivity towards trypsin digestion of soluble scFv suggested that the lack of binding activity was caused by incorrect folding of Q6E mutants. Grafting of the three additional class IA derived FR1 residues, based upon the comparison between both classes of VH sequences, on to the 'defect' subgroup IIA sequence, partially restored the antigen binding activity of the Q6E-containing scFv. Our results suggest that residue 6 of the heavy chain may be part of a folding nucleus, involving the first two beta-strands of Framework region 1. The evolutionary conservation of either glutamine or glutamate at position 6 in different antibody families may well indicate that within immunoglobulin VH domains, different family specific folding nuclei have evolved.lld:pubmed
pubmed-article:9930677pubmed:languageenglld:pubmed
pubmed-article:9930677pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:citationSubsetIMlld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9930677pubmed:statusMEDLINElld:pubmed
pubmed-article:9930677pubmed:monthDeclld:pubmed
pubmed-article:9930677pubmed:issn0269-2139lld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:BoenderPPlld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:HoogenboomH...lld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:ArendsJ WJWlld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:van der...lld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:KazemierBBlld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:van GemenBBlld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:OudshoornPPlld:pubmed
pubmed-article:9930677pubmed:authorpubmed-author:de HaardH JHJlld:pubmed
pubmed-article:9930677pubmed:issnTypePrintlld:pubmed
pubmed-article:9930677pubmed:volume11lld:pubmed
pubmed-article:9930677pubmed:ownerNLMlld:pubmed
pubmed-article:9930677pubmed:authorsCompleteYlld:pubmed
pubmed-article:9930677pubmed:pagination1267-76lld:pubmed
pubmed-article:9930677pubmed:dateRevised2011-11-17lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:meshHeadingpubmed-meshheading:9930677-...lld:pubmed
pubmed-article:9930677pubmed:year1998lld:pubmed
pubmed-article:9930677pubmed:articleTitleAbsolute conservation of residue 6 of immunoglobulin heavy chain variable regions of class IIA is required for correct folding.lld:pubmed
pubmed-article:9930677pubmed:affiliationBiosciences Research Unit, Organon Teknika, Boxtel, The Netherlands. hans-de.haard@unilever.comlld:pubmed
pubmed-article:9930677pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9930677pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9930677lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9930677lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9930677lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9930677lld:pubmed