| pubmed-article:9923451 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9923451 | lifeskim:mentions | umls-concept:C0205252 | lld:lifeskim |
| pubmed-article:9923451 | lifeskim:mentions | umls-concept:C0040052 | lld:lifeskim |
| pubmed-article:9923451 | lifeskim:mentions | umls-concept:C0025166 | lld:lifeskim |
| pubmed-article:9923451 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:9923451 | lifeskim:mentions | umls-concept:C0332197 | lld:lifeskim |
| pubmed-article:9923451 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9923451 | pubmed:dateCreated | 1999-2-25 | lld:pubmed |
| pubmed-article:9923451 | pubmed:abstractText | We examined withdrawal effects of recombinant mouse Tpo (rm-Tpo) on the apoptosis of mature and immature megakaryocytes in in vitro experiments. Apoptotic megakaryocytes were detected by double staining for acetylcholinesterase and by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. When the purified mature megakaryocytes were cultured with or without rm-Tpo, the numbers of viable megakaryocytes, apoptotic megakaryocytes, and megakaryocytes with cytoplasmic processes were not significantly different between the two groups. In contrast, purified immature megakaryocytes underwent apoptosis when rm-Tpo was absent from the culture system. Murine bone marrow cells were cultured with rm-Tpo (50 U/mL) on days 1-7 to generate immature megakaryocytes and subsequently were cultured with different concentrations of rm-Tpo (0-50 U/mL) on days 8-14. The number of viable megakaryocytes was decreased and that of apoptotic megakaryocytes was increased by rm-Tpo in a dose-dependent manner. These results indicated a clear relation between the rm-Tpo level and the apoptosis of immature megakaryocytes. | lld:pubmed |
| pubmed-article:9923451 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9923451 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9923451 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9923451 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9923451 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9923451 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9923451 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:9923451 | pubmed:issn | 0301-472X | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:AbeTT | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:KojimaHH | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:NagasawaTT | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:TodokoroKK | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:TakizawaMM | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:NinomiyaHH | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:OsadaMM | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:SuzukawaKK | lld:pubmed |
| pubmed-article:9923451 | pubmed:author | pubmed-author:KomenoTT | lld:pubmed |
| pubmed-article:9923451 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9923451 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:9923451 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9923451 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9923451 | pubmed:pagination | 131-8 | lld:pubmed |
| pubmed-article:9923451 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9923451 | pubmed:meshHeading | pubmed-meshheading:9923451-... | lld:pubmed |
| pubmed-article:9923451 | pubmed:meshHeading | pubmed-meshheading:9923451-... | lld:pubmed |
| pubmed-article:9923451 | pubmed:meshHeading | pubmed-meshheading:9923451-... | lld:pubmed |
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| pubmed-article:9923451 | pubmed:meshHeading | pubmed-meshheading:9923451-... | lld:pubmed |
| pubmed-article:9923451 | pubmed:meshHeading | pubmed-meshheading:9923451-... | lld:pubmed |
| pubmed-article:9923451 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:9923451 | pubmed:articleTitle | Immature megakaryocytes undergo apoptosis in the absence of thrombopoietin. | lld:pubmed |
| pubmed-article:9923451 | pubmed:affiliation | Division of Hematology, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan. | lld:pubmed |
| pubmed-article:9923451 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9923451 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9923451 | lld:pubmed |
