pubmed-article:9882374 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C0220847 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C1749467 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C0035685 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
pubmed-article:9882374 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:9882374 | pubmed:issue | 2 | lld:pubmed |
pubmed-article:9882374 | pubmed:dateCreated | 1999-2-18 | lld:pubmed |
pubmed-article:9882374 | pubmed:abstractText | Production of soluble full-length nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) has been shown to be problematic and requires the addition of salts, glycerol, and detergents. In an effort to improve the solubility of NS5B, the hydrophobic C terminus containing 21 amino acids was removed, yielding a truncated NS5B (NS5BDeltaCT) which is highly soluble and monodispersed in the absence of detergents. Fine deletional analysis of this region revealed that a four-leucine motif (LLLL) in the hydrophobic domain is responsible for the solubility profile of the full-length NS5B. Enzymatic characterization revealed that the RNA-dependent RNA polymerase (RdRp) activity of this truncated NS5B was comparable to those reported previously by others. For optimal enzyme activity, divalent manganese ions (Mn2+) are preferred rather than magnesium ions (Mg2+), whereas zinc ions (Zn2+) inhibit the RdRp activity. Gliotoxin, a known poliovirus 3D RdRp inhibitor, inhibited HCV NS5B RdRp in a dose-dependent manner. Kinetic analysis revealed that HCV NS5B has a rather low processivity compared to those of other known polymerases. | lld:pubmed |
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pubmed-article:9882374 | pubmed:language | eng | lld:pubmed |
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pubmed-article:9882374 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:9882374 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:9882374 | pubmed:month | Feb | lld:pubmed |
pubmed-article:9882374 | pubmed:issn | 0022-538X | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:FerrariEE | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:BaroudyB MBM | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:LinJ HJH | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:HongZZ | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:FangJ WJW | lld:pubmed |
pubmed-article:9882374 | pubmed:author | pubmed-author:Wright-Minogu... | lld:pubmed |
pubmed-article:9882374 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:9882374 | pubmed:volume | 73 | lld:pubmed |
pubmed-article:9882374 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:9882374 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:9882374 | pubmed:pagination | 1649-54 | lld:pubmed |
pubmed-article:9882374 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:9882374 | pubmed:year | 1999 | lld:pubmed |
pubmed-article:9882374 | pubmed:articleTitle | Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. | lld:pubmed |
pubmed-article:9882374 | pubmed:affiliation | Antiviral Therapy, Schering-Plough Research Institute, Kenilworth, New Jersey 07033-0539, USA. | lld:pubmed |
pubmed-article:9882374 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:9882374 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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