| pubmed-article:9843964 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0009337 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0040690 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0162493 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0919432 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C1333079 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C1413592 | lld:lifeskim |
| pubmed-article:9843964 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:9843964 | pubmed:issue | 25 | lld:pubmed |
| pubmed-article:9843964 | pubmed:dateCreated | 1999-1-14 | lld:pubmed |
| pubmed-article:9843964 | pubmed:abstractText | The human type VII collagen gene (COL7A1) recently has been identified as an immediate-early response gene for transforming growth factor beta (TGF-beta)/SMAD signaling pathway. In this study, by using MDA-MB-468 SMAD4-/- breast carcinoma cells, we demonstrate that expression of SMAD4 is an absolute requirement for SMAD-mediated promoter activity. We also demonstrate that the SMAD binding sequence (SBS) representing the TGF-beta response element in the region -496/-444 of the COL7A1 promoter functions as an enhancer in the context of a heterologous promoter. Electrophoretic mobility-shift assays with nuclear extracts from COS-1 cells transfected with expression vectors for SMADs 1-5 indicate that SMAD3 forms a complex with a migration similar to that of the endogenous TGF-beta-specific complex observed in fibroblast extracts. Electrophoretic mobility-shift assays using recombinant glutathione S-transferase-SMAD fusion proteins indicate that both SMAD4 and C-terminally truncated SMAD3, but not SMAD2, can bind the COL7A1 SBS. Coexpression of SMAD3 and SMAD4 in COS-1 cells leads to the formation of two complexes: a DNA/protein complex containing SMAD3 alone and another slower-migrating complex containing both SMAD3 and SMAD4, the latter complex not being detected in fibroblasts. Maximal transactivation of COL7A1 SBS-driven promoters in either MDA-MB-468 carcinoma cells or fibroblasts requires concomitant overexpression of SMAD3 and SMAD4. These data may represent the first identification of a functional homomeric SMAD3 complex regulating a human gene. | lld:pubmed |
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| pubmed-article:9843964 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:9843964 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9843964 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9843964 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9843964 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:UittoJJ | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:KonAA | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:RobertsA BAB | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:MauvielAA | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:LechleiderR... | lld:pubmed |
| pubmed-article:9843964 | pubmed:author | pubmed-author:VindevoghelLL | lld:pubmed |
| pubmed-article:9843964 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9843964 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:9843964 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:9843964 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9843964 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9843964 | pubmed:pagination | 14769-74 | lld:pubmed |
| pubmed-article:9843964 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9843964 | pubmed:meshHeading | pubmed-meshheading:9843964-... | lld:pubmed |
| pubmed-article:9843964 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9843964 | pubmed:articleTitle | SMAD3/4-dependent transcriptional activation of the human type VII collagen gene (COL7A1) promoter by transforming growth factor beta. | lld:pubmed |
| pubmed-article:9843964 | pubmed:affiliation | Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA. | lld:pubmed |
| pubmed-article:9843964 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9843964 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9843964 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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