| pubmed-article:9826331 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0445750 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0042765 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0449943 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C0599946 | lld:lifeskim |
| pubmed-article:9826331 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:9826331 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:9826331 | pubmed:dateCreated | 1998-12-24 | lld:pubmed |
| pubmed-article:9826331 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9826331 | pubmed:abstractText | Salmonella enterica poxA mutants exhibit a pleiotropic phenotype, including reduced pyruvate oxidase activity; reduced growth rate; and hypersensitivity to the herbicide sulfometuron methyl, alpha-ketobutyrate, and amino acid analogs. These mutants also failed to grow in the presence of the host antimicrobial peptide, protamine. In this study, PoxA- mutants of S. enterica serovar Typhimurium (S. typhimurium) were found to be 10,000-fold attenuated in orally inoculated BALB/c mice and 1,000-fold attenuated in intraperitoneally inoculated BALB/c mice, compared to wild-type S. typhimurium UK-1. In addition, poxA mutants were found to be capable of colonizing the spleen, mesenteric lymph nodes, and Peyer's patches; to induce strong humoral immune responses; and to protect mice against a lethal wild-type Salmonella challenge. A 2-kb DNA fragment was isolated from wild-type S. typhimurium UK-1 based on its ability to complement an isogenic poxA mutant. The nucleotide sequence of this DNA fragment revealed an open reading frame of 325 amino acids capable of encoding a polypeptide of 36.8 kDa that was confirmed in the bacteriophage T7 expression system. Comparison of the translated sequence to the available databases indicated high homology to a family of lysyl-tRNA synthetases. Our results indicate that a mutation of poxA has an attenuating effect on Salmonella virulence. Further, poxA mutants are immunogenic and could be useful in designing live vaccines with a variety of bacterial species. To our knowledge, this is the first report on the effect of poxA mutation on bacterial virulence. | lld:pubmed |
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| pubmed-article:9826331 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9826331 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9826331 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9826331 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9826331 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9826331 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9826331 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:9826331 | pubmed:author | pubmed-author:SundaramPP | lld:pubmed |
| pubmed-article:9826331 | pubmed:author | pubmed-author:CurtissRR3rd | lld:pubmed |
| pubmed-article:9826331 | pubmed:author | pubmed-author:KanigaKK | lld:pubmed |
| pubmed-article:9826331 | pubmed:author | pubmed-author:ComptonM SMS | lld:pubmed |
| pubmed-article:9826331 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9826331 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:9826331 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9826331 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9826331 | pubmed:pagination | 5599-606 | lld:pubmed |
| pubmed-article:9826331 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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