| pubmed-article:9820842 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9820842 | lifeskim:mentions | umls-concept:C0206679 | lld:lifeskim |
| pubmed-article:9820842 | lifeskim:mentions | umls-concept:C0017968 | lld:lifeskim |
| pubmed-article:9820842 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
| pubmed-article:9820842 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:9820842 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:9820842 | pubmed:dateCreated | 1999-2-1 | lld:pubmed |
| pubmed-article:9820842 | pubmed:abstractText | Based on sequence analysis, the protein encoded by the US5 open reading frame (ORF) of herpes simplex virus type 1 (HSV-1) was predicted to contain an N-glycosylation site and was given the designation of glycoprotein J (gJ). However, the US5 gene product has not been identified and the identity of gJ as a glycoprotein has not been confirmed. We have cloned and expressed the DNA encoding the complete sequence of the US5 ORF, using a baculovirus expression system. Western blotting, using polyclonal antibody raised against synthetic US5 peptides, revealed two major baculovirus-US5-expressed protein bands with apparent molecular weights of 16-17 and 10 kD. The recombinant US5 was found on the membrane of Spodoptera frugiperda cells and was susceptible to tunicamycin, endoglycosidase H, glycosidase F and partially resistant to endoglycosidase F. Vaccination of mice with baculovirus-expressed US5 did not induce a neutralizing antibody to HSV-1 or provide protection against lethal HSV-1 challenge. However, serum from these vaccinated mice was able to recognize US5 in purified HSV-1 virions by Western blot analyses and on the surface of HSV-1-infected cells by immunofluorescence. These findings establish that US5 does encode a glycoprotein and confirm the appropriateness of naming the US5 gene product gJ. | lld:pubmed |
| pubmed-article:9820842 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9820842 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9820842 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9820842 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9820842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9820842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9820842 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9820842 | pubmed:issn | 0300-5526 | lld:pubmed |
| pubmed-article:9820842 | pubmed:author | pubmed-author:NesburnA BAB | lld:pubmed |
| pubmed-article:9820842 | pubmed:author | pubmed-author:WechslerS LSL | lld:pubmed |
| pubmed-article:9820842 | pubmed:author | pubmed-author:CapJJ | lld:pubmed |
| pubmed-article:9820842 | pubmed:author | pubmed-author:GhiasiHH | lld:pubmed |
| pubmed-article:9820842 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9820842 | pubmed:volume | 41 | lld:pubmed |
| pubmed-article:9820842 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9820842 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9820842 | pubmed:pagination | 91-7 | lld:pubmed |
| pubmed-article:9820842 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9820842 | pubmed:meshHeading | pubmed-meshheading:9820842-... | lld:pubmed |
| pubmed-article:9820842 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9820842 | pubmed:articleTitle | The US5 open reading frame of herpes simplex virus type 1 does encode a glycoprotein (gJ). | lld:pubmed |
| pubmed-article:9820842 | pubmed:affiliation | Ophthalmology Research, Cedars-Sinai Medical Center Research Institute, Los Angeles, Calif., 90048, USA. ghiasi@csmc.edu | lld:pubmed |
| pubmed-article:9820842 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9820842 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9820842 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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