| pubmed-article:9820826 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0040715 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0007009 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0035147 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1522702 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1413190 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C2698901 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1422405 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1422515 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0599718 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0599813 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0599893 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9820826 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9820826 | pubmed:dateCreated | 1999-1-26 | lld:pubmed |
| pubmed-article:9820826 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9820826 | pubmed:abstractText | The human BTG1 protein is thought to be a potential tumour suppressor because its overexpression inhibits NIH 3T3 cell proliferation. However, little is known about how BTG1 exerts its anti-proliferative activity. In this study, we used the yeast 'two-hybrid' system to screen for interacting protein partners and identified human carbon catabolite repressor protein (CCR4)-associative factor 1 (hCAF-1), a homologue of mouse CAF-1 (mCAF-1) and Saccharomyces cerevisiae yCAF-1/POP2. In vitro the hCAF-1/BTG1 complex formation was dependent on the phosphorylation of a putative p34cdc2 kinase site on BTG1 (Ser-159). In yeast, the Ala-159 mutant did not interact with hCAF-1. In addition, phosphorylation of Ser-159 in vitro showed specificity for the cell cycle kinases p34CDK2/cyclin E and p34CDK2/cyclin A, but not for p34CDK4/cyclin D1 or p34cdc2/cyclin B. Cell synchrony experiments with primary cultures of rat aortic smooth-muscle cells (RSMCs) demonstrated that message and protein levels of rat CAF-1 (rCAF-1) were up-regulated under conditions of cell contact, as previously reported for BTG1 [Wilcox, Scott, Subramanian, Ross, Adams-Burton, Stoltenborg and Corjay (1995) Circulation 92, I34-I35]. Western blot and immunohistochemical analysis showed that rCAF-1 localizes to the nucleus of contact-inhibited RSMCs, where it was physically associated with BTG1, as determined by co-immunoprecipitation with anti-hCAF-1 antisera. Overexpression of hCAF-1 in NIH 3T3 and osteosarcoma (U-2-OS) cells was itself anti-proliferative with colony formation reduced by 67% and 90% respectively. Taken together, these results indicate that formation of the hCAF-1/BTG1 complex is driven by phosphorylation at BTG1 (Ser-159) and implicates this complex in the signalling events of cell division that lead to changes in cellular proliferation associated with cell-cell contact. | lld:pubmed |
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| pubmed-article:9820826 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9820826 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9820826 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9820826 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9820826 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9820826 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:9820826 | pubmed:author | pubmed-author:BenfieldP APA | lld:pubmed |
| pubmed-article:9820826 | pubmed:author | pubmed-author:DickerI BIB | lld:pubmed |
