| pubmed-article:9815132 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9815132 | lifeskim:mentions | umls-concept:C0687028 | lld:lifeskim |
| pubmed-article:9815132 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:9815132 | lifeskim:mentions | umls-concept:C1882714 | lld:lifeskim |
| pubmed-article:9815132 | lifeskim:mentions | umls-concept:C0213238 | lld:lifeskim |
| pubmed-article:9815132 | lifeskim:mentions | umls-concept:C1516698 | lld:lifeskim |
| pubmed-article:9815132 | pubmed:issue | 5 Pt 2 | lld:pubmed |
| pubmed-article:9815132 | pubmed:dateCreated | 1998-12-10 | lld:pubmed |
| pubmed-article:9815132 | pubmed:abstractText | Vesicle targeting proteins ("SNAREs") have been proposed to direct vasopressin-induced trafficking of aquaporin-2 water channels in kidney collecting ducts. A newly identified SNARE protein, SNAP-23, is proposed to mediate vesicle targeting to the plasma membrane in diverse tissues. The current studies were done to determine whether SNAP-23 is expressed in collecting ducts with an intracellular distribution compatible with a role in aquaporin-2 trafficking. RT-PCR demonstrated SNAP-23 mRNA in microdissected collecting ducts and other tubular segments including the proximal tubule and thick ascending limb. Immunoblotting using a polyclonal antibody raised against a COOH-terminal peptide revealed a solitary band at an apparent molecular mass of 30 kDa in renal medullary membrane fractions and inner medullary collecting duct suspensions. Differential centrifugation revealed that SNAP-23 is present in membrane fractions including the low-density fraction enriched in intracellular vesicles. Immunocytochemistry revealed SNAP-23 labeling at both the apex and the cytoplasm of collecting duct principal cells. Immunoblotting of intracellular vesicles immunoisolated using an aquaporin-2 antibody revealed the presence of both SNAP-23 and synaptobrevin-2 (VAMP-2) in aquaporin-2-bearing vesicles. We conclude that SNAP-23 is strongly expressed in collecting duct principal cells, consistent with a role in vasopressin-regulated trafficking of aquaporin-2. However, localization of SNAP-23 in both intracytoplasmic vesicles and plasma membranes suggests a function different from that originally proposed for SNAP-25 in synaptic vesicle targeting. | lld:pubmed |
| pubmed-article:9815132 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9815132 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9815132 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9815132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9815132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9815132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9815132 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9815132 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9815132 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9815132 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:InoueTT | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:NielsenSS | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:KnepperM AMA | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:KishoreB KBK | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:TerrisJJ | lld:pubmed |
| pubmed-article:9815132 | pubmed:author | pubmed-author:MandonBB | lld:pubmed |
| pubmed-article:9815132 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9815132 | pubmed:volume | 275 | lld:pubmed |
| pubmed-article:9815132 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9815132 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9815132 | pubmed:pagination | F752-60 | lld:pubmed |
| pubmed-article:9815132 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9815132 | pubmed:meshHeading | pubmed-meshheading:9815132-... | lld:pubmed |
| pubmed-article:9815132 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9815132 | pubmed:articleTitle | SNAP-23 in rat kidney: colocalization with aquaporin-2 in collecting duct vesicles. | lld:pubmed |
| pubmed-article:9815132 | pubmed:affiliation | Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1603, USA. | lld:pubmed |
| pubmed-article:9815132 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9815132 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9815132 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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