| pubmed-article:9813208 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C0026200 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1135858 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C0028326 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C0002520 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1706044 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1415222 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1552866 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C2700399 | lld:lifeskim |
| pubmed-article:9813208 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:9813208 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9813208 | pubmed:dateCreated | 1998-12-17 | lld:pubmed |
| pubmed-article:9813208 | pubmed:abstractText | GST-NS1 purified from Escherichia coli and insect cells binds double-strand DNA in an (ACCA)2-3-dependent fashion under similar ionic conditions, independent of the presence of anti-NS1 antisera or exogenously supplied ATP and interacts with single-strand DNA and RNA in a sequence-independent manner. An amino-terminal domain (amino acids 1-275) of NS1 [GST-NS1(1-275)], representing 41% of the full-length NS1 molecule, includes a domain that binds double-strand DNA in a sequence-specific manner at levels comparable to full-length GST-NS1, as well as single-strand DNA and RNA in a sequence-independent manner. The deletion of 15 additional amino-terminal amino acids yielded a molecule [GST-NS1(1-275)] that maintained (ACCA)2-3-specific double-strand DNA binding; however, this molecule was more sensitive to increasing ionic conditions than full-length GST-NS1 and GST-NS1(1-275) and could not be demonstrated to bind single-strand nucleic acids. A quantitative filter binding assay showed that E. coli- and baculovirus-expressed GST-NS1 and E. coli GST-NS1(1-275) specifically bound double-strand DNA with similar equilibrium kinetics [as measured by their apparent equilibrium DNA binding constants (KD)], whereas GST-NS1(16-275) bound 4- to 8-fold less well. | lld:pubmed |
| pubmed-article:9813208 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9813208 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9813208 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9813208 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9813208 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:9813208 | pubmed:author | pubmed-author:MoréPP | lld:pubmed |
| pubmed-article:9813208 | pubmed:author | pubmed-author:PintelD JDJ | lld:pubmed |
| pubmed-article:9813208 | pubmed:copyrightInfo | Copyright 1998 Academic Press. | lld:pubmed |
| pubmed-article:9813208 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9813208 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:9813208 | pubmed:volume | 251 | lld:pubmed |
| pubmed-article:9813208 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9813208 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9813208 | pubmed:pagination | 123-31 | lld:pubmed |
| pubmed-article:9813208 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:meshHeading | pubmed-meshheading:9813208-... | lld:pubmed |
| pubmed-article:9813208 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9813208 | pubmed:articleTitle | Amino acids 16-275 of minute virus of mice NS1 include a domain that specifically binds (ACCA)2-3-containing DNA. | lld:pubmed |
| pubmed-article:9813208 | pubmed:affiliation | School of Medicine, University of Missouri, Columbia, Missouri, 65212, USA. | lld:pubmed |
| pubmed-article:9813208 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9813208 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9813208 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9813208 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9813208 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9813208 | lld:pubmed |
