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pubmed-article:9809475pubmed:abstractText1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.lld:pubmed
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pubmed-article:9809475pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9809475pubmed:articleTitleEffects of basic fibroblast growth factor on proliferation and phenotype expression of chondrocytes embedded in collagen gel.lld:pubmed
pubmed-article:9809475pubmed:affiliationDepartment of Orthopaedics, Shimane Medical University, Japan.lld:pubmed
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