Linked Life Data

9806868

Source:http://linkedlifedata.com/resource/pubmed/id/9806868

Statements in which the resource exists.
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pubmed-article:9806868pubmed:abstractTextA 1.6-kb tandem repeat sequence had previously been identified in the subtelomeric region of mini- and megabase chromosomes from Leishmania braziliensis. Southern hybridisation was used to demonstrate that the repeat is complex specific. The sequence was characterised in strains representing four species of the L. braziliensis complex. This data allowed an assessment of the evolutionary relationship of the four species. PCR primers targeted to the repeat amplify only DNA from species of the L. braziliensis complex. Titration assays indicate that a minimum of 50 fg of parasite DNA can be detected by PCR alone. Southern hybridisation increases the limit of detection to 5 fg. Interspecies variation in the repeat sequence enabled restriction enzyme digestion of PCR products to distinguish individual species within the L. braziliensis complex.lld:pubmed
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pubmed-article:9806868pubmed:copyrightInfoCopyright 1998 Academic Press.lld:pubmed
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pubmed-article:9806868pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9806868pubmed:articleTitleLeishmania braziliensis: characterisation of a complex specific subtelomeric repeat sequence and its use in the detection of parasites.lld:pubmed
pubmed-article:9806868pubmed:affiliationMRC Outstation of NIMR, Molteno Laboratories, Department of Pathology, University of Cambridge, CB2 1QP, U.K.lld:pubmed
pubmed-article:9806868pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9806868pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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