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pubmed-article:9802014pubmed:abstractTextSecreted aspartic proteinases (Saps) contribute to the virulence of Candida albicans in systemic animal models of infection. Seven genes encoding Saps (SAP1-SAP7) have been identified to date but evidence suggested the existence of additional SAP genes. The screening of a C. albicans lambda EMBL3 genomic library for the presence of other SAP genes was undertaken. Two new genes, SAP8 and SAP9, were isolated. The N-terminal amino acid sequence deduced from SAP8 downstream of a Kex2p-like cleavage site corresponds to the N-terminal amino acid sequence of the 41 kDa Sap isolated and characterized previously. SAP8 mRNA was expressed preferentially in yeasts at 25 degrees C after 6 and 9 h growth in BSA-containing medium. SAP9 encodes an aspartic proteinase with a Kex2p-like cleavage site and contains a putative glycophosphatidylinositol-anchor signal at the C-terminus. Although the SAP9 gene product has not yet been isolated from cultures of C. albicans, transcripts of SAP9 were observed preferentially in later growth phases when SAP8 expression had decreased.lld:pubmed
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pubmed-article:9802014pubmed:articleTitleDifferential regulation of SAP8 and SAP9, which encode two new members of the secreted aspartic proteinase family in Candida albicans.lld:pubmed
pubmed-article:9802014pubmed:affiliationService de Dermatologie (DHURDV), Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland. Michel.Monod@chuv.hospvd.chlld:pubmed
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