| pubmed-article:9801074 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9801074 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:9801074 | lifeskim:mentions | umls-concept:C0225698 | lld:lifeskim |
| pubmed-article:9801074 | lifeskim:mentions | umls-concept:C0016693 | lld:lifeskim |
| pubmed-article:9801074 | lifeskim:mentions | umls-concept:C0239059 | lld:lifeskim |
| pubmed-article:9801074 | lifeskim:mentions | umls-concept:C0599732 | lld:lifeskim |
| pubmed-article:9801074 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9801074 | pubmed:dateCreated | 1999-1-15 | lld:pubmed |
| pubmed-article:9801074 | pubmed:abstractText | Cigarette smoke augments asbestos-induced bronchogenic carcinoma by mechanisms that are not established. Alveolar epithelial cell (AEC) injury due to oxidant-induced DNA damage and depletion of glutathione (GSH) and adenosine triphosphate (ATP) may be one important mechanism. We previously showed that amosite asbestos-induces hydroxyl radical production and DNA damage to cultured AEC and that phytic acid, an iron chelator, is protective. We hypothesized that whole cigarette smoke extracts (CSE) augment amosite asbestos-induced AEC injury by generating iron-induced free radicals that damage DNA and reduce cellular GSH and ATP levels. Asbestos or CSE each caused dose-dependent toxicity to AEC (WI-26 and rat alveolar type I-like cells) as assessed by 51chromium release. The combination of asbestos (5 microg/cm2) and CSE (0.O1-0.1%) caused synergistic injury whereas higher doses of each agent primarily had an additive toxic effect. Asbestos (5 microg/cm2) augmented CSE-induced (0.01-1.0%) AEC DNA damage over a 4 h exposure period as assessed by an alkaline unwinding, ethidium bromide fluorometric technique. These effects were synergistic in A549 cells and additive in WI-26 cells. Asbestos (5 microg/cm2) and CSE (0.5-1.0%) reduced A549 and WI-26 cell GSH levels as assessed spectrophotometrically and ATP levels as assessed by luciferin/luciferase chemiluminescence but a synergistic interaction was not detected. Phytic acid (500 microM) and catalase (100 microg/ml) each attenuated A549 cell DNA damage and depletion of ATP caused by asbestos and CSE. However, neither agent attenuated WI-26 cell DNA damage nor the reductions in GSH levels in WI-26 and A549 cells exposed to asbestos and CSE. We conclude that CSE enhance asbestos-induced DNA damage in cultured alveolar epithelial cells. These data provide additional support that asbestos and cigarette smoke are genotoxic to relevant target cells in the lung and that iron-induced free radicals may in part cause these effects. | lld:pubmed |
| pubmed-article:9801074 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9801074 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9801074 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9801074 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9801074 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9801074 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9801074 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9801074 | pubmed:issn | 0891-5849 | lld:pubmed |
| pubmed-article:9801074 | pubmed:author | pubmed-author:WeitzmanS ASA | lld:pubmed |
| pubmed-article:9801074 | pubmed:author | pubmed-author:KammD PDP | lld:pubmed |
| pubmed-article:9801074 | pubmed:author | pubmed-author:GreenbergerM... | lld:pubmed |
| pubmed-article:9801074 | pubmed:author | pubmed-author:PreusenS ESE | lld:pubmed |
| pubmed-article:9801074 | pubmed:author | pubmed-author:SbalchierroJ... | lld:pubmed |
| pubmed-article:9801074 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9801074 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:9801074 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9801074 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9801074 | pubmed:pagination | 728-39 | lld:pubmed |
| pubmed-article:9801074 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9801074 | pubmed:meshHeading | pubmed-meshheading:9801074-... | lld:pubmed |
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| pubmed-article:9801074 | pubmed:meshHeading | pubmed-meshheading:9801074-... | lld:pubmed |
| pubmed-article:9801074 | pubmed:meshHeading | pubmed-meshheading:9801074-... | lld:pubmed |
| pubmed-article:9801074 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9801074 | pubmed:articleTitle | Cigarette smoke augments asbestos-induced alveolar epithelial cell injury: role of free radicals. | lld:pubmed |
| pubmed-article:9801074 | pubmed:affiliation | Department of Medicine, Northwestern University Medical School, Veterans Affairs Chicago Health Care System, IL 60611, USA. d-kamp@nwu.edu | lld:pubmed |
| pubmed-article:9801074 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9801074 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:9801074 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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