| pubmed-article:9795276 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9795276 | lifeskim:mentions | umls-concept:C0017622 | lld:lifeskim |
| pubmed-article:9795276 | lifeskim:mentions | umls-concept:C0002518 | lld:lifeskim |
| pubmed-article:9795276 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:9795276 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:9795276 | pubmed:dateCreated | 1999-1-25 | lld:pubmed |
| pubmed-article:9795276 | pubmed:abstractText | We have determined the partial amino acid sequence (207 amino acids) of gamma-46 gliadin isolated from wheat cultivar Hardi. The molecular mass of the protein (Mr) estimated by electrospray mass spectrometry is 35191.3. The number of cysteine residues in gamma-46 gliadin was determined as a mass difference of the protein before and after reduction and alkylation with 4-vinylpyridine. It was shown that the protein has no free SH-groups, and all cysteine residues are involved in the formation of four disulfide bonds. The partial structure of gamma-46 gliadin was determined by N-terminal sequencing and sequencing of tryptic and chymotryptic peptides. The tryptic peptides were obtained by enzymatic hydrolysis of the protein, which was preliminarily reduced and immobilized at free SH-groups on thiopropyl-Sepharose 6B. The chymotryptic peptides were isolated by limited digestion of the native protein. The positions of cysteine residues, as well as surrounding amino acid sequences, are conserved in gamma-46 gliadin; this is typical of gliadins. | lld:pubmed |
| pubmed-article:9795276 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9795276 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9795276 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9795276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9795276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9795276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9795276 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9795276 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9795276 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9795276 | pubmed:issn | 0006-2979 | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:RoepstorffPP | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:PopineauYY | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:AndersenJJ | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:EgorovT ATA | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:BarbashovS... | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:OdintsovaT... | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:PustobaevV... | lld:pubmed |
| pubmed-article:9795276 | pubmed:author | pubmed-author:MusolyamovA... | lld:pubmed |
| pubmed-article:9795276 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9795276 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:9795276 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9795276 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9795276 | pubmed:pagination | 1061-7 | lld:pubmed |
| pubmed-article:9795276 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:meshHeading | pubmed-meshheading:9795276-... | lld:pubmed |
| pubmed-article:9795276 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9795276 | pubmed:articleTitle | Partial amino acid sequence of gamma-46 gliadin. | lld:pubmed |
| pubmed-article:9795276 | pubmed:affiliation | Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984, Russia. egorov@imb.imb.ac.ru. | lld:pubmed |
| pubmed-article:9795276 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9795276 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
