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| pubmed-article:9790664 | lifeskim:mentions | umls-concept:C0205431 | lld:lifeskim |
| pubmed-article:9790664 | pubmed:issue | 43 | lld:pubmed |
| pubmed-article:9790664 | pubmed:dateCreated | 1998-11-17 | lld:pubmed |
| pubmed-article:9790664 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9790664 | pubmed:abstractText | The cell wall of the Gram-positive bacterium Corynebacterium glutamicum contains a channel (porin) for the passage of hydrophilic solutes. The channel-forming protein was identified, by lipid bilayer experiments, in the cell envelope fractions isolated by sucrose-density centrifugations and in organic solvent of whole cells. It was purified to homogeneity by fast-protein liquid chromatography across a Mono-Q column. The pure protein had a rather low molecular mass of about 5 kDa as judged by SDS-PAGE, which suggested that the cell wall channel is formed by a protein oligomer. The monomer has according to partial sequencing no significant homology to known protein sequences. The purified protein formed large ion-permeable channels in lipid bilayer membranes from phosphatidylcholine/phosphatidylserine mixtures with a single-channel conductance of 5.5 nS in 1 M KCl. Experiments with different salts suggested that the cell wall channel of C. glutamicum was highly cation-selective caused by negative charges localized at the channel mouth. The analysis of the single-channel conductance data using the Renkin correction factor suggested that the diameter of the cell wall channel is about 2.2 nm. Channel-forming properties of the cell wall channel of C. glutamicum were compared with those of mycobacteria. These channels share common features because they form large and water-filled channels that contain point net charges. | lld:pubmed |
| pubmed-article:9790664 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9790664 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9790664 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9790664 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9790664 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9790664 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:9790664 | pubmed:author | pubmed-author:BenzRR | lld:pubmed |
| pubmed-article:9790664 | pubmed:author | pubmed-author:KrämerRR | lld:pubmed |
| pubmed-article:9790664 | pubmed:author | pubmed-author:NiederweisMM | lld:pubmed |
| pubmed-article:9790664 | pubmed:author | pubmed-author:BurkovskiAA | lld:pubmed |
| pubmed-article:9790664 | pubmed:author | pubmed-author:LichtingerTT | lld:pubmed |
| pubmed-article:9790664 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9790664 | pubmed:day | 27 | lld:pubmed |
| pubmed-article:9790664 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:9790664 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9790664 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9790664 | pubmed:pagination | 15024-32 | lld:pubmed |
| pubmed-article:9790664 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9790664 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9790664 | pubmed:articleTitle | Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: the channel is formed by a low molecular mass polypeptide. | lld:pubmed |
| pubmed-article:9790664 | pubmed:affiliation | Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Germany. | lld:pubmed |
| pubmed-article:9790664 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9790664 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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