| pubmed-article:9787113 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0086574 | lld:lifeskim |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0031715 | lld:lifeskim |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0072402 | lld:lifeskim |
| pubmed-article:9787113 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:9787113 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9787113 | pubmed:dateCreated | 1998-12-3 | lld:pubmed |
| pubmed-article:9787113 | pubmed:abstractText | We have previously shown that apoptosis induced in thymocytes by dexamethasone or teniposide (VM-26) could be inhibited by 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7) and sangivamycin, both relatively specific inhibitors for protein kinase C, but not by N-(2-guanidinoethyl)-5-isoquinolinesulfonamide (HA1004), a more specific inhibitor for cAMP-dependent protein kinases. Apoptosis in this model system was not blocked by EGTA and no increase in cytosolic Ca2+ was observed during apoptosis induced by either dexamethasone or VM-26, suggesting that this kinase was Ca2+-independent. In the present study, we demonstrate that addition of 10 microM sangivamycin to thymocyte cultures up to 2 h after addition of either inducer resulted in virtually complete inhibition of apoptosis. Addition of 10 microM sangivamycin at 3 or 4 h after addition of inducer resulted in partial inhibition of apoptosis. Computerized image analysis of two-dimensional PAGE analyses of whole-cell lysates demonstrated that treatment of mouse thymocytes with VM-26 resulted in a limited number of de novo phosphorylation events within 1 h of treatment. The most prominent phosphorylation events associated with VM-26-induced apoptosis were that two intracellular protein species (Protein 1: m.w. = 22.9 kDa, pI, 5.11; and Protein 2: m.w. = 22.9 kDa, pI, 4.98). Similar phosphorylation events were seen in cells treated with dexamethasone. Finally, Western blot analysis suggests that de novo protein phosphorylation induced by VM-26 is on serine/threonine residues. These results provide further evidence that the mechanism of VM-26-induced apoptosis of murine thymocytes involves the action of one or more serine/threonine kinases. | lld:pubmed |
| pubmed-article:9787113 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9787113 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9787113 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9787113 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9787113 | pubmed:issn | 0090-1229 | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:CohenSS | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:YuHH | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:CoffmanF DFD | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:FresaK LKL | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:HingleyS TST | lld:pubmed |
| pubmed-article:9787113 | pubmed:author | pubmed-author:ModyN SNS | lld:pubmed |
| pubmed-article:9787113 | pubmed:copyrightInfo | Copyright 1998 Academic Press. | lld:pubmed |
| pubmed-article:9787113 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9787113 | pubmed:volume | 89 | lld:pubmed |
| pubmed-article:9787113 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9787113 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9787113 | pubmed:pagination | 117-25 | lld:pubmed |
| pubmed-article:9787113 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
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| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:meshHeading | pubmed-meshheading:9787113-... | lld:pubmed |
| pubmed-article:9787113 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9787113 | pubmed:articleTitle | Protein phosphorylation associated with epipodophyllotoxin-induced apoptosis of lymphoid cells: role of a serine/threonine protein kinase. | lld:pubmed |
| pubmed-article:9787113 | pubmed:affiliation | Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, 19131, USA. | lld:pubmed |
| pubmed-article:9787113 | pubmed:publicationType | Journal Article | lld:pubmed |
