Linked Life Data

9778250

Source:http://linkedlifedata.com/resource/pubmed/id/9778250

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pubmed-article:9778250pubmed:abstractTextErythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 (BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.lld:pubmed
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pubmed-article:9778250pubmed:articleTitleA SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro.lld:pubmed
pubmed-article:9778250pubmed:affiliationRegulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.lld:pubmed
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pubmed-article:9778250pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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