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pubmed-article:9776816pubmed:abstractTextTo analyse if hydrocortisone could modify the in vitro susceptibility of Aspergillus fumigatus to antifungal drugs, we developed a procedure to test the susceptibility of an A. fumigatus strain to amphotericin B and itraconazole, grown in the presence and in the absence of hydrocortisone. Conidia were germinated in the presence or the absence of hydrocortisone in Czapek medium without antifungal drug. A dilution of these cultures (5x10(3) conidia ml(-1)) was spread onto Czapek-agarose plates containing both antifungal drug and hydrocortisone. The cfu per plate were enumerated and compared. A therapeutic concentration of hydrocortisone induced a significant increase in the susceptibility to itraconazole. Conversely, the susceptibility to amphotericin B was not significantly modified when this antifungal drug was associated with hydrocortisone.lld:pubmed
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pubmed-article:9776816pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9776816pubmed:articleTitleDoes hydrocortisone modify the in vitro susceptibility of Aspergillus fumigatus to itraconazole and amphotericin B?lld:pubmed
pubmed-article:9776816pubmed:affiliationDépartement de Parasitologie-Mycologie Médicale et Moléculaire, CNRS UPRES-A 5082, Faculté de Médecine et de Pharmacie, Université Joseph Fourier, Grenoble I. La Tronche, France. Isabelle. Ramondenc@ujfgrenoble.frlld:pubmed
pubmed-article:9776816pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9776816pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed