| pubmed-article:9763212 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0225828 | lld:lifeskim |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0034785 | lld:lifeskim |
| pubmed-article:9763212 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9763212 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:9763212 | pubmed:dateCreated | 1998-10-15 | lld:pubmed |
| pubmed-article:9763212 | pubmed:abstractText | Previous work indicated that hyperstimulation of muscarinic receptors brings about profound changes not only in the density of the muscarinic receptors, but also of the beta-adrenoceptors in rat heart atria in vivo. We have now investigated whether a similar receptor cross-regulation occurs in cardiomyocytes in vitro. Cardiomyocytes from 3-4 day old rats were exposed to chemical agents on days 5-6 in culture. Densities of muscarinic and beta-adrenergic receptors were measured according to the binding of N-[3H]methylscopolamine and [ H]CGP 12177, respectively, to cell surface membranes and cell homogenates. Exposure of cells to the muscarinic agonist carbachol (1 mmol/l) brought about a profound decrease in the number of muscarinic receptors. The number of beta-adrenoceptors displayed biphasic changes, being augmented after 24 h (by 20-45% on the cell surface and by 29% in the homogenate) and diminished after 48 h and 72 h (after 48 h, decrease by 44-75% on the cell surface and by 36% in the homogenate). These effects of carbachol were not prevented by dimethylaminopropyl-bis-indolylmaleimide, the inhibitor of protein kinase C. Exposure of cells to the beta-adrenoceptor agonist isoprenaline (0.1 mmol/l) strongly diminished the number of beta-adrenoceptors on the cell surface and in the homogenate. The density of muscarinic receptors on the cell surface was diminished by 24-43% after 24 h exposure to isoprenaline and unchanged after 48 h, whereas the concentration of muscarinic receptors in the homogenate was unchanged after 24 h and increased by 20% after 48 h. The isoprenaline-induced decrease in the density of cell surface muscarinic receptors could not be simulated by forskolin and was not abolished by the protein kinase A inhibitors Rp-cAMPS and HA-1004. Dibutyryl cyclic AMP diminished the density of cell surface muscarinic receptors more than that of the beta-adrenergic receptors. Our data reveal a novel phenomenon of a biphasic change (an increase followed by a loss) in the density of beta-adrenoceptors during exposure of cardiocytes to carbachol. Activation of beta-adrenoceptors brings about less conspicuous changes in the density of muscarinic receptors. The observed phenomena of receptor cross-regulation cannot be explained by simple activations of protein kinases A and C. | lld:pubmed |
| pubmed-article:9763212 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9763212 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9763212 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9763212 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:9763212 | pubmed:author | pubmed-author:MyslivecekJJ | lld:pubmed |
| pubmed-article:9763212 | pubmed:author | pubmed-author:TucekSS | lld:pubmed |
| pubmed-article:9763212 | pubmed:author | pubmed-author:NoelE PEP | lld:pubmed |
| pubmed-article:9763212 | pubmed:author | pubmed-author:TrojanSS | lld:pubmed |
| pubmed-article:9763212 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9763212 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:9763212 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9763212 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9763212 | pubmed:pagination | 1169-82 | lld:pubmed |
| pubmed-article:9763212 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:9763212 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9763212 | pubmed:articleTitle | Heterologous regulation of muscarinic and beta-adrenergic receptors in rat cardiomyocytes in culture. | lld:pubmed |
| pubmed-article:9763212 | pubmed:affiliation | Institute of Physiology, Academy of Sciences of the Czech Republic, Prague. | lld:pubmed |
| pubmed-article:9763212 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9763212 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9763212 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
