| pubmed-article:9760277 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0003062 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0440744 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0242958 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:9760277 | lifeskim:mentions | umls-concept:C1980032 | lld:lifeskim |
| pubmed-article:9760277 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:9760277 | pubmed:dateCreated | 1998-11-9 | lld:pubmed |
| pubmed-article:9760277 | pubmed:abstractText | Malondialdehyde (MDA) is a major lipid peroxidation product that is mutagenic and tumorigenic. The MDA-modified DNA adduct, 3-(2-deoxy-beta-D-erythro-pentofuranosyl)pyrimido[1, 2-alpha]purin-10(3H)-one (M1G), has been detected in human tissues and may be a marker of human cancer risk. In this paper, we describe an improved 32P-postlabeling/HPLC method for sensitive detection and quantitation of this MDA-modified 2'-deoxyribonucleotide adduct. Specific improvements include (i) unequivocal structural identification of the postlabeling products, both the 3', 5'-bisphosphate of M1G (MDA-3',5'-dGDP) and the 5'-monophosphate of M1G (MDA-5'-dGMP); (ii) efficient separation of the 32P-postlabeling products by HPLC; and (iii) the incorporation of a synthetically prepared MDA-modified DNA (or the 3'-monophosphate of M1G) with a known modification level as an internal standard. This improved quantitative methodology provides high intra- and inter-assay reproducibility and has been applied to the analysis of this adduct in rodent and human samples. | lld:pubmed |
| pubmed-article:9760277 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9760277 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9760277 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9760277 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9760277 | pubmed:issn | 0893-228X | lld:pubmed |
| pubmed-article:9760277 | pubmed:author | pubmed-author:FuP PPP | lld:pubmed |
| pubmed-article:9760277 | pubmed:author | pubmed-author:DoergeD RDR | lld:pubmed |
| pubmed-article:9760277 | pubmed:author | pubmed-author:SudVV | lld:pubmed |
| pubmed-article:9760277 | pubmed:author | pubmed-author:YiPP | lld:pubmed |
| pubmed-article:9760277 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9760277 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:9760277 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9760277 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9760277 | pubmed:pagination | 1032-41 | lld:pubmed |
| pubmed-article:9760277 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
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| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
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| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:meshHeading | pubmed-meshheading:9760277-... | lld:pubmed |
| pubmed-article:9760277 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9760277 | pubmed:articleTitle | An improved 32P-postlabeling/high-performance liquid chromatography method for the analysis of the malondialdehye-derived 1, N2-propanodeoxyguanosine DNA adduct in animal and human tissues. | lld:pubmed |
| pubmed-article:9760277 | pubmed:affiliation | National Center for Toxicological Research, Jefferson, Arkansas 72079, USA. | lld:pubmed |
| pubmed-article:9760277 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9760277 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9760277 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9760277 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9760277 | lld:pubmed |
