| pubmed-article:9719487 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0010934 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0206131 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0299583 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:9719487 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:9719487 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:9719487 | pubmed:dateCreated | 1998-9-8 | lld:pubmed |
| pubmed-article:9719487 | pubmed:abstractText | A greater understanding of the factors causing the enhanced release of leptin by adipocytes in obesity is needed. Experiments were designed to determine the effects of actinomycin D on leptin release by isolated rat adipocytes during primary culture for 24 hr. In adipocytes from fed hypothyroid rats, the initial rate of leptin release over the first 6 hr was not maintained over the next 18 hr. The decline in leptin release by adipocytes in primary culture between 6 and 24 hr was reduced markedly by either dexamethasone or actinomycin D. Both actinomycin D and dexamethasone also reduced the loss of leptin mRNA seen over the 24-hr incubation. Maximal effects on leptin release and leptin mRNA accumulation required only 0.1 microM of actinomycin D, a concentration that had no significant effect on the 18S RNA content of adipocytes at the end of a 24-hr incubation. In contrast to the reduced loss of leptin mRNA seen at 24 hr, the loss of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid (GAPDH mRNA) was enhanced in the presence of 0.1 microM of actinomycin D. The effects of dexamethasone could be differentiated from those of actinomycin D by the finding that cycloheximide blocked the reduced loss of leptin mRNA due to dexamethasone while having no effect on that due to actinomycin D. These results point to a unique regulation of leptin release and leptin mRNA levels by actinomycin D. | lld:pubmed |
| pubmed-article:9719487 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9719487 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9719487 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9719487 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9719487 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:9719487 | pubmed:author | pubmed-author:FainJ NJN | lld:pubmed |
| pubmed-article:9719487 | pubmed:author | pubmed-author:BahouthS WSW | lld:pubmed |
| pubmed-article:9719487 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9719487 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9719487 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:9719487 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9719487 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9719487 | pubmed:pagination | 1309-14 | lld:pubmed |
| pubmed-article:9719487 | pubmed:dateRevised | 2003-11-14 | lld:pubmed |
| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
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| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
| pubmed-article:9719487 | pubmed:meshHeading | pubmed-meshheading:9719487-... | lld:pubmed |
| pubmed-article:9719487 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9719487 | pubmed:articleTitle | Stimulation of leptin release by actinomycin D in rat adipocytes. | lld:pubmed |
| pubmed-article:9719487 | pubmed:affiliation | Department of Biochemistry, College of Medicine, University of Tennessee at Memphis, 38163, USA. jfain@utmem1.utmem.edu | lld:pubmed |
| pubmed-article:9719487 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9719487 | lld:pubmed |
