| pubmed-article:9717969 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0034821 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0229664 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0836921 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9717969 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9717969 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:9717969 | pubmed:dateCreated | 1998-9-9 | lld:pubmed |
| pubmed-article:9717969 | pubmed:abstractText | B lymphocytes, purified from peripheral leucocytes from young normolipaemic humans, expressed and internalized low-density lipoprotein receptors (LDLR). The expression was assessed by a monoclonal anti-LDLR. The internalization of LDL was assessed by LDL labelled with 125I (125I-LDL) and 1,1'-dioctadecyl-3,3,3',3' tetramethyl-indocarboxycyanine perchlorate (LDL-DiI). The expression of LDLR, assessed by anti-LDLR, was: 38 +/- 8% (n = 5) for fresh purified cells, 60 +/- 10% (n = 12) for non-stimulated cells, 79 +/- 5% (n = 10) for IL-2 (100 U/ml)-stimulated cells and 95 +/- 5% (n = 8) for pokeweed mitogen (PWM) (1:200 dilution)-stimulated cells. The optimal concentrations of agonist were 100 U/ml of IL-2, and 1:200 dilution of PWM. IL-2 and PWM increased the internalization of LDL-DiI by 1.5-fold. The internalization of LDL-DiI was maximal at 60 microg of protein/ml (48 +/- 8%). Scatchard analysis revealed a Kd of 3.2 +/- 0.22 x 10(-8) M and 2180 +/- 190 binding sites in non-stimulated cells, a Kd of 7.73 +/- 0.36 x 10(-9) M and 12,500 +/- 430 binding sites for IL-2 (100 U/ml)-stimulated cells, and a Kd of 7.2 +/- 0.43 x 10(-9) M and 13,250 +/- 450 binding sites for PWM (1:200 dilution)-stimulated cells. Lineweaver-Burk analysis of LDL binding (LDL-DiI) revealed that the apparent Kd for non-stimulated cells was 1.3 +/- 0.11 x 10(-8) M, and 9.2 +/- 0.2 x 10(-9) M and 7.5 +/- 0.25 x 10(-9) M for IL-2- and PWM-stimulated cells, respectively. B lymphocytes from tonsils also showed a high expression of LDLR assessed with anti-LDLR (70 +/- 6%). The high expression of LDLR and the avid internalization of LDL suggest that LDL may be important for B cell physiological responses. | lld:pubmed |
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| pubmed-article:9717969 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9717969 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9717969 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9717969 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9717969 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9717969 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9717969 | pubmed:issn | 0009-9104 | lld:pubmed |
| pubmed-article:9717969 | pubmed:author | pubmed-author:BiancoN ENE | lld:pubmed |
| pubmed-article:9717969 | pubmed:author | pubmed-author:BlanckCC | lld:pubmed |
| pubmed-article:9717969 | pubmed:author | pubmed-author:RiveraHH | lld:pubmed |
| pubmed-article:9717969 | pubmed:author | pubmed-author:De SanctisJ... | lld:pubmed |
| pubmed-article:9717969 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9717969 | pubmed:volume | 113 | lld:pubmed |
| pubmed-article:9717969 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9717969 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9717969 | pubmed:pagination | 206-12 | lld:pubmed |
| pubmed-article:9717969 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:9717969 | pubmed:meshHeading | pubmed-meshheading:9717969-... | lld:pubmed |
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| pubmed-article:9717969 | pubmed:meshHeading | pubmed-meshheading:9717969-... | lld:pubmed |
| pubmed-article:9717969 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9717969 | pubmed:articleTitle | Expression of low-density lipoprotein receptors in peripheral blood and tonsil B lymphocytes. | lld:pubmed |
| pubmed-article:9717969 | pubmed:affiliation | Institute of Immunology, Faculty of Medicine, Central University of Venezuela, Caracas. | lld:pubmed |
| pubmed-article:9717969 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9717969 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
