| pubmed-article:9693131 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0282639 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0026682 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0332157 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0205161 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C1417497 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:9693131 | lifeskim:mentions | umls-concept:C0205355 | lld:lifeskim |
| pubmed-article:9693131 | pubmed:dateCreated | 1998-10-8 | lld:pubmed |
| pubmed-article:9693131 | pubmed:abstractText | Previous work has shown that treatment of HT-29 methotrexate (MTX) cells with benzyl-N-acetyl-alpha-D-galactosaminide results in profound changes in mucin oligosaccharide chains. To analyse in depth the effect of this drug, we first determined the structure of mucin oligosaccharide chains synthesized by HT-29 MTX cells and the changes induced by permanent drug exposure. Mucins from untreated cells contained nine monosialylated structures (core types 1, 2, 3 and 4) and four disialylated structures (types 1, 2 and 4). Core 1 structures predominated, in particular NeuAcalpha2-3Galbeta1-3GalNAc-ol. Exposure of HT-29 MTX cells to benzyl-N-acetyl-alpha-D-galactosaminide from days 2-21 resulted in a decrease in intracellular mucins and both their sialic acid and galactose content, and an increased T (Galbeta1-3GalNAcalpha-O-Ser/Thr) and Tn (GalNAcalpha-O-Ser/Thr) antigenicity. A 3-fold increase in both Galbeta1-3GalNAc alpha2, 3-sialyltransferase activity and mRNA expression was detected. At the ultrastructural level, T-antigen was not detectable in mucin droplets in control cells, but was strongly expressed in intracytoplasmic vesicles in treated cells. In these cells, MUC1 and MUC3 transcripts were up-regulated, whereas MUC2, MUC5B and MUC5AC were down-regulated. Furthermore, constitutive and secretagogue-induced MUC5AC secretion was reduced and no mucus layer was detected. In conclusion, benzyl-N-acetyl-alpha-D-galactosaminide induces abnormal O-glycosylation and altered regulation of MUC5AC secretion. | lld:pubmed |
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| pubmed-article:9693131 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9693131 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9693131 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9693131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9693131 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9693131 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9693131 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:DelannoyPP | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:AubertJ PJP | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:RichetCC | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:DegandPP | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:MoreauOO | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:ZweibaumAA | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:KiiMM | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:TingNN | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:HuetGG | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:CaponCC | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:LesuffleurTT | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:HémonBB | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:RecchiM AMA | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:De BolosCC | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:Hennebicq-Rei... | lld:pubmed |
| pubmed-article:9693131 | pubmed:author | pubmed-author:MaësEE | lld:pubmed |
| pubmed-article:9693131 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9693131 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9693131 | pubmed:volume | 334 ( Pt 1) | lld:pubmed |
| pubmed-article:9693131 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9693131 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9693131 | pubmed:pagination | 283-95 | lld:pubmed |
| pubmed-article:9693131 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:9693131 | pubmed:meshHeading | pubmed-meshheading:9693131-... | lld:pubmed |
| pubmed-article:9693131 | pubmed:meshHeading | pubmed-meshheading:9693131-... | lld:pubmed |
| pubmed-article:9693131 | pubmed:meshHeading | pubmed-meshheading:9693131-... | lld:pubmed |
