| pubmed-article:9689816 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9689816 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9689816 | lifeskim:mentions | umls-concept:C0032000 | lld:lifeskim |
| pubmed-article:9689816 | lifeskim:mentions | umls-concept:C0033371 | lld:lifeskim |
| pubmed-article:9689816 | lifeskim:mentions | umls-concept:C0169964 | lld:lifeskim |
| pubmed-article:9689816 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:9689816 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:9689816 | pubmed:dateCreated | 1998-9-8 | lld:pubmed |
| pubmed-article:9689816 | pubmed:abstractText | Co-localization of human growth hormone (hGH) and human prolactin (hPRL) in hGH-producing pituitary adenomas was examined by electron microscopy with immunoblot analysis. At the electron microscopic level using anti-hGH or anti-hPRL polyclonal antibody, hGH and hPRL were found to be co-localized within each of the secretory granules in one of five cases. Double-labeling electron immunocytochemistry using colloidal gold particles of different sizes was effective in demonstrating this co-localization. As an additional step, we performed immunoblot analysis of hGH-producing pituitary adenomas using monoclonal antibodies. Four hGH-producing adenomatous tissue samples contained several hPRL-immunoreactive bands. In Case 2, the main 23K hPRL band was stained especially strongly The immunoblotting analysis of purified hGH using both anti-hPRL polyclonal antibody and monoclonal antibody to asses cross-reaction of the polyclonal anti-hPRL antisera with hGH revealed that both monoclonal and polyclonal antibodies were suitable for determining the co-localization. Double-labeling techniques using anti-hGH and anti-hPRL monoclonal antibodies demonstrated that only a few secretory granules were positive for co-localization of both hGH and hPRL (Case 2). The present study, which used not only polyclonal but also monoclonal antibodies, suggests that some hGH-producing pituitary adenomas contained both hPRL and hGH in the same secretory granules of tumor cells. | lld:pubmed |
| pubmed-article:9689816 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9689816 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9689816 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9689816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9689816 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9689816 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9689816 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9689816 | pubmed:issn | 0470-8105 | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:WatanabeTT | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:HiranoHH | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:HiraiKK | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:KawakamiHH | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:HoriTT | lld:pubmed |
| pubmed-article:9689816 | pubmed:author | pubmed-author:KurosakiMM | lld:pubmed |
| pubmed-article:9689816 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9689816 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:9689816 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9689816 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9689816 | pubmed:pagination | 335-9; discussion 340-1 | lld:pubmed |
| pubmed-article:9689816 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
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| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:meshHeading | pubmed-meshheading:9689816-... | lld:pubmed |
| pubmed-article:9689816 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9689816 | pubmed:articleTitle | Co-localization of human growth hormone and human prolactin in hormonally pure human growth hormone-producing pituitary adenomas. | lld:pubmed |
| pubmed-article:9689816 | pubmed:affiliation | Division of Neurosurgery, Institute of Neurological Sciences, Tottori University School of Medicine, Tokyo. | lld:pubmed |
| pubmed-article:9689816 | pubmed:publicationType | Journal Article | lld:pubmed |
