| pubmed-article:9680061 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0020205 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0376436 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0035191 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0205195 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:9680061 | lifeskim:mentions | umls-concept:C2346972 | lld:lifeskim |
| pubmed-article:9680061 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9680061 | pubmed:dateCreated | 1998-10-26 | lld:pubmed |
| pubmed-article:9680061 | pubmed:abstractText | A library system was developed for the discovery of bioactive peptides. Library synthesis and peptide sequencing was performed on a solid support while the screening for bioactivity was done with peptides in solution. The peptides were synthesized by split and mix, one-bead-one-peptide library synthesis, using a Tentagel S-NH2 solid support with a loading of approximately 100 pmol/bead. The major part of the peptide was connected to the support by a single acid-labile linker and a minor part of the peptide was acid-stabile attached to the polymer. The percentage of acid-stabile attached peptides could easily be controlled during modification of the amino functionalities of the resin at the start of the process. The cleavage rate of the acid-labile attached peptide from the resin depends on the composition of the cleavage mixture. When cleavage conditions were carefully controlled, a three-step partial cleavage protocol allowed for convergent bioactivity screening on peptide libraries using only one type of acid-labile linker. The partial cleavage and convergent screening procedure was repeated three times, after which the bead containing the bioactive peptide was sequenced. As such a bead still contained acid-stabile attached peptide, the Edman sequencing was straightforward and repetitive yields were excellent because the immobilized peptide was not washed out. | lld:pubmed |
| pubmed-article:9680061 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9680061 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9680061 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9680061 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9680061 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9680061 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9680061 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9680061 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:9680061 | pubmed:issn | 1075-2617 | lld:pubmed |
| pubmed-article:9680061 | pubmed:author | pubmed-author:AmonsRR | lld:pubmed |
| pubmed-article:9680061 | pubmed:author | pubmed-author:RappWW | lld:pubmed |
| pubmed-article:9680061 | pubmed:author | pubmed-author:DrijfhoutJ... | lld:pubmed |
| pubmed-article:9680061 | pubmed:author | pubmed-author:BenckhuijsenW... | lld:pubmed |
| pubmed-article:9680061 | pubmed:author | pubmed-author:HiemstraH SHS | lld:pubmed |
| pubmed-article:9680061 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9680061 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:9680061 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9680061 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9680061 | pubmed:pagination | 282-8 | lld:pubmed |
| pubmed-article:9680061 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:meshHeading | pubmed-meshheading:9680061-... | lld:pubmed |
| pubmed-article:9680061 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9680061 | pubmed:articleTitle | A new hybrid resin for stepwise screening of peptide libraries combined with single bead Edman sequencing. | lld:pubmed |
| pubmed-article:9680061 | pubmed:affiliation | Department of Immunohematology and Blood Bank, Leiden University Hospital, The Netherlands. | lld:pubmed |
| pubmed-article:9680061 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9680061 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9680061 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9680061 | lld:pubmed |
