| pubmed-article:9675111 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9675111 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:9675111 | lifeskim:mentions | umls-concept:C0017374 | lld:lifeskim |
| pubmed-article:9675111 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9675111 | lifeskim:mentions | umls-concept:C0598991 | lld:lifeskim |
| pubmed-article:9675111 | lifeskim:mentions | umls-concept:C0449445 | lld:lifeskim |
| pubmed-article:9675111 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9675111 | pubmed:dateCreated | 1998-8-6 | lld:pubmed |
| pubmed-article:9675111 | pubmed:abstractText | Synthetic genes are very useful in genetic and protein engineering. Here we propose a general method for construction of synthetic genes. Short oligonucleotides are joined through ligase chain reaction (LCR) in high stringency conditions to make "unit fragments" which are then fused to form a full-length gene sequence by polymerase chain reaction. The procedure is simple and accurate and does not place constraints on sequence and length. In this report, a recombinant leptin gene was synthesized according to the codon preference of Escherichia coli. Besides, a substitution of the only Met at position 54 for Leu and an addition of a Met at the N-terminus were introduced in the synthetic gene. The gene was cloned in the pQE-31 expression vector and was expressed in E. coli. A large amount of recombinant leptin containing 6 x His tag was produced and purified by Ni-NTA affinity column. Finally, intact leptin-L54 was released after removing the tag by CNBr cleavage at the Met residue. | lld:pubmed |
| pubmed-article:9675111 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9675111 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9675111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9675111 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9675111 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:9675111 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:9675111 | pubmed:author | pubmed-author:LaoAA | lld:pubmed |
| pubmed-article:9675111 | pubmed:author | pubmed-author:YangW JWJ | lld:pubmed |
| pubmed-article:9675111 | pubmed:author | pubmed-author:ONOHH | lld:pubmed |
| pubmed-article:9675111 | pubmed:author | pubmed-author:YangF YFY | lld:pubmed |
| pubmed-article:9675111 | pubmed:author | pubmed-author:KauC LCL | lld:pubmed |
| pubmed-article:9675111 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9675111 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:9675111 | pubmed:volume | 248 | lld:pubmed |
| pubmed-article:9675111 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9675111 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9675111 | pubmed:pagination | 200-3 | lld:pubmed |
| pubmed-article:9675111 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9675111 | pubmed:meshHeading | pubmed-meshheading:9675111-... | lld:pubmed |
| pubmed-article:9675111 | pubmed:meshHeading | pubmed-meshheading:9675111-... | lld:pubmed |
| pubmed-article:9675111 | pubmed:meshHeading | pubmed-meshheading:9675111-... | lld:pubmed |
| pubmed-article:9675111 | pubmed:meshHeading | pubmed-meshheading:9675111-... | lld:pubmed |
| pubmed-article:9675111 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9675111 | pubmed:articleTitle | Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli. | lld:pubmed |
| pubmed-article:9675111 | pubmed:affiliation | Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, Republic of China. lcau@isc1.vghtpe.gov.tw | lld:pubmed |
| pubmed-article:9675111 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9675111 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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