Linked Life Data

9661662

Source:http://linkedlifedata.com/resource/pubmed/id/9661662

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pubmed-article:9661662pubmed:abstractTextThe reverse transcription polymerase chain reaction (RT-PCR) with primers specific for each of the 14 exons of the human complement regulatory protein membrane cofactor protein (MCP;CD46) has been utilized to determine MCP mRNA transcript expression in peripheral blood mononuclear cells (PBMC). An additional transcript of a larger size than predicted was consistently detected in reactions with a sense primer for exon 7, that encodes the first alternatively spliced serine-threonine-rich region (ST-A), together with an antisense exon 12 primer, RT-PCR with primers for other exons both 5' and 3' of exon 7 further showed that these MCP transcripts contain additional sequences immediately both 5' and 3' to the exon 7-encoded sequence. Comparison of genomic DNA with cDNA by PCR, in combination with sequence analysis, demonstrated the presence of the complete invariant sequences of both introns adjacent to exon 7, i.e. intron 6 (411 bp) and intron 7 (127 bp). RT-PCR using primers specific for the intron 6 sequence, together with Southern and Northern blotting using an intron 6-specific probe, confirmed retention of this intron within a novel 4.8-kb mRNA transcript in human PBMC. Due to the presence of a stop codon within intron 6, translation would result in a novel truncated MCP isoform (MCPi) containing the four invariant short consensus repeat (SCR) regions and a unique C-terminal 39 amino acid transmembrane and cytoplasmic tail region that may promote endoplasmic reticulum retention.lld:pubmed
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pubmed-article:9661662pubmed:authorpubmed-author:JohnsonP MPMlld:pubmed
pubmed-article:9661662pubmed:authorpubmed-author:NewtonD JDJlld:pubmed
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pubmed-article:9661662pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9661662pubmed:articleTitleA novel isoform of human membrane cofactor protein (CD46) mRNA generated by intron retention.lld:pubmed
pubmed-article:9661662pubmed:affiliationDepartment of Immunology, University of Liverpool, UK.lld:pubmed
pubmed-article:9661662pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9661662pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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