| pubmed-article:9633997 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C0024485 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:9633997 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:9633997 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:9633997 | pubmed:dateCreated | 1998-7-2 | lld:pubmed |
| pubmed-article:9633997 | pubmed:abstractText | The 19F NMR resonances of intra- and extracellular 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil (L-FMAU) in suspensions of human erythrocytes are well resolved. This phenomenon allows its transport behavior to be monitored in a 19F NMR time-course experiment. The rate of L-FMAU uptake at 25 degrees in a suspension containing L-FMAU at an initial extracellular concentration of 4 mM was 7.6 +/- 1.0 x 10(-7) pmol cell(-1) sec(-1) (N = 5). Concentration-dependent uptake studies of L-FMAU indicate the existence of both saturable and nonsaturable transport mechanisms, with a Km for the saturable uptake of approximately 1 mM. Although the transport of L-FMAU at 25 degrees was inhibited significantly (54-65%) by nitrobenzylthioinosine (NBTI) and dipyridamole, consistent with the participation of the nucleoside transporter, these inhibitors did not achieve complete blockage of L-FMAU uptake. The participation of the nucleobase transporter in L-FMAU uptake was ruled out by the absence of competition with uracil uptake, and by the lack of inhibition by papaverine. In addition, the NBTI-insensitive uptake of L-FMAU was not affected by pretreatment of the cells with the sulfhydryl reagent, p-chloromercuriphenylsulfonic acid (pCMBS). However, the NBTI- and dipyridamole-insensitive transport of L-FMAU was found to increase upon treatment of the erythrocytes with butanol, an agent that affects membrane fluidity. The partition coefficient of L-FMAU in octanol/phosphate-buffered saline determined by absorption spectrophotometry was 0.31. These data indicate that under the conditions of the studies, L-FMAU uptake by erythrocytes proceeds by both the nucleoside transporter and nonfacilitated membrane diffusion. | lld:pubmed |
| pubmed-article:9633997 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9633997 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9633997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9633997 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9633997 | pubmed:month | May | lld:pubmed |
| pubmed-article:9633997 | pubmed:issn | 0006-2952 | lld:pubmed |
| pubmed-article:9633997 | pubmed:author | pubmed-author:LondonR ERE | lld:pubmed |
| pubmed-article:9633997 | pubmed:author | pubmed-author:ChiC WCW | lld:pubmed |
| pubmed-article:9633997 | pubmed:author | pubmed-author:XuA SAS | lld:pubmed |
| pubmed-article:9633997 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9633997 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9633997 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:9633997 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9633997 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9633997 | pubmed:pagination | 1611-9 | lld:pubmed |
| pubmed-article:9633997 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:meshHeading | pubmed-meshheading:9633997-... | lld:pubmed |
| pubmed-article:9633997 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9633997 | pubmed:articleTitle | 19F NMR study of the uptake of 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil in erythrocytes: evidence of transport by facilitated and nonfacilitated pathways. | lld:pubmed |
| pubmed-article:9633997 | pubmed:affiliation | Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709-2233, USA. | lld:pubmed |
| pubmed-article:9633997 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9633997 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
