| pubmed-article:9600842 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9600842 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:9600842 | lifeskim:mentions | umls-concept:C2349183 | lld:lifeskim |
| pubmed-article:9600842 | lifeskim:mentions | umls-concept:C1879748 | lld:lifeskim |
| pubmed-article:9600842 | lifeskim:mentions | umls-concept:C1706853 | lld:lifeskim |
| pubmed-article:9600842 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9600842 | pubmed:dateCreated | 1998-6-16 | lld:pubmed |
| pubmed-article:9600842 | pubmed:abstractText | X-ray fiber diffraction is potentially powerful in solving the atomic structure of filamentous assemblies of macromolecules, as demonstrated for tobacco mosaic virus. However, it requires extremely well-oriented sols to allow for extraction of intensities on closely located layer-lines. A high degree of orientation requires a high filament concentration to restrain the orientational freedom, but orienting concentrated sols is hampered by their high viscosity. Here, we report a systematic method that reproducibly produces extremely good orientation, which involves three steps; liquid crystallization, centrifugation and magnetic orientation. We found that a slow centrifugation can trigger a dynamic self-orientation process to form perfectly homogeneous liquid-crystalline sols, and further centrifugation to concentrate sols followed by magnetic orientation produces exceptionally well-oriented sols. The best-oriented flagellar sol showed a disorientation angle of 0.6 degrees as 1sigma of its Gaussian distribution. The new method has been successfully applied to many other systems, such as tobacco mosaic virus and F-actin. | lld:pubmed |
| pubmed-article:9600842 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9600842 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9600842 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9600842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9600842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9600842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9600842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9600842 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9600842 | pubmed:month | May | lld:pubmed |
| pubmed-article:9600842 | pubmed:issn | 0022-2836 | lld:pubmed |
| pubmed-article:9600842 | pubmed:author | pubmed-author:NambaKK | lld:pubmed |
| pubmed-article:9600842 | pubmed:author | pubmed-author:SuzukiHH | lld:pubmed |
| pubmed-article:9600842 | pubmed:author | pubmed-author:YamashitaII | lld:pubmed |
| pubmed-article:9600842 | pubmed:copyrightInfo | Copyright 1998 Academic Press Limited. | lld:pubmed |
| pubmed-article:9600842 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9600842 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:9600842 | pubmed:volume | 278 | lld:pubmed |
| pubmed-article:9600842 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9600842 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9600842 | pubmed:pagination | 609-15 | lld:pubmed |
| pubmed-article:9600842 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:meshHeading | pubmed-meshheading:9600842-... | lld:pubmed |
| pubmed-article:9600842 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9600842 | pubmed:articleTitle | Multiple-step method for making exceptionally well-oriented liquid-crystalline sols of macromolecular assemblies. | lld:pubmed |
| pubmed-article:9600842 | pubmed:affiliation | International Institute for Advanced Research, Matsushita Electric Industrial Co., Ltd, 3-4 Hikaridai, Seika, 619-02, Japan. | lld:pubmed |
| pubmed-article:9600842 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9600842 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9600842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9600842 | lld:pubmed |
