| pubmed-article:9546673 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0030946 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0483249 | lld:lifeskim |
| pubmed-article:9546673 | lifeskim:mentions | umls-concept:C0599013 | lld:lifeskim |
| pubmed-article:9546673 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9546673 | pubmed:dateCreated | 1998-5-13 | lld:pubmed |
| pubmed-article:9546673 | pubmed:abstractText | A high-affinity RNA aptamer to human activated protein C (APC) was selected from a pool of random sequences using in vitro selection. Activated protein C, a trypsin-like serine protease plays an important role along with thrombin as a regulator in blood clotting cascade. After seven rounds of selection and amplification, a single predominant nucleic acid sequence APC-167, a 167-base oligonucleotide with a random sequence core of 120 bases, was obtained. The selected aptamer did not bind to thrombin or factor Xa and thus demonstrated specificity to APC. Furthermore, this aptamer was a non-competitive inhibitor to the cleavage reaction of a fluorogenic substrate catalyzed by APC. The inhibition constant (Ki) of APC-167 was 83 nM. The 99-base oligonucleotide (APC-99) derived from APC-167 by deleting both primer binding sites, was also found to inhibit APC strongly (Ki = 137 nM). Two stem-loop structures and at least one G x U wobble base pair in the stem were elucidated as important structural motifs for binding. | lld:pubmed |
| pubmed-article:9546673 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9546673 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9546673 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9546673 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9546673 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9546673 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:NishikawaSS | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:NishikawaFF | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:GayS TST | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:KumarP KPK | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:Vishnuvardhan... | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:AmontovSS | lld:pubmed |
| pubmed-article:9546673 | pubmed:author | pubmed-author:UrvilP TPT | lld:pubmed |
| pubmed-article:9546673 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9546673 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9546673 | pubmed:volume | 252 | lld:pubmed |
| pubmed-article:9546673 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9546673 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9546673 | pubmed:pagination | 553-62 | lld:pubmed |
| pubmed-article:9546673 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
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| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:meshHeading | pubmed-meshheading:9546673-... | lld:pubmed |
| pubmed-article:9546673 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9546673 | pubmed:articleTitle | Selection of a RNA aptamer that binds to human activated protein C and inhibits its protease function. | lld:pubmed |
| pubmed-article:9546673 | pubmed:affiliation | National Institute of Bioscience and Human Technology, AIST, MITI, Tsukuba Science City, Ibaraki, Japan. | lld:pubmed |
| pubmed-article:9546673 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9546673 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9546673 | lld:pubmed |
