Linked Life Data

9546382

Source:http://linkedlifedata.com/resource/pubmed/id/9546382

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:9546382rdf:typepubmed:Citationlld:pubmed
pubmed-article:9546382lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0228174lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0025962lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C1545588lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0265110lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0205217lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0596235lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0439799lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0332120lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C2911684lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0185117lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0332324lld:lifeskim
pubmed-article:9546382lifeskim:mentionsumls-concept:C0857121lld:lifeskim
pubmed-article:9546382pubmed:issue6lld:pubmed
pubmed-article:9546382pubmed:dateCreated1998-4-24lld:pubmed
pubmed-article:9546382pubmed:abstractTextThe Ca2+-sensitive K+ channel (K(Ca) channel) plays a key role in buffering pressure-induced constriction of small cerebral arteries. An amplified current through this channel has been reported in vascular smooth muscle cells obtained from hypertensive animals, implying that the expression or properties of K(Ca) channels may be regulated by in vivo blood pressure levels. In this study, we investigated this hypothesis and its functional relevance by comparing the properties, expression levels, and physiological role of K(Ca) channels in cerebral resistance arteries from normotensive and genetically hypertensive rats. Whole-cell patch-clamp experiments revealed a 4.7-fold higher density of iberiotoxin-sensitive K(Ca) channel current at physiological membrane potentials in spontaneously hypertensive rat (SHR) compared with Wistar-Kyoto (WKY) rat cerebrovascular smooth muscle cells (n = 18 and 21, respectively). However, additional single-channel analysis in detached patches showed similar levels of unitary conductance, voltage, and Ca2+ sensitivity in K(Ca) channels from WKY and from SHR membranes. In contrast, Western analysis using an antibody directed against the K(Ca) channel alpha-subunit revealed a 4.1-fold increase in the corresponding 125-kD immunoreactive signal in cerebrovascular membranes from SHR compared with WKY rats. The functional impact of this enhanced K(Ca) channel expression was assessed in SHR and WKY rat pial arterioles, which were monitored by intravital microscopy through in situ cranial windows. Progressive pharmacological block of K(Ca) channels by iberiotoxin (0.1 to 100 nmol/L) dose-dependently constricted pial arterioles from SHR and WKY rats (n = 6 to 8). The arterioles in SHR constricted 2- to 4-fold more intensely, and vasospasm occurred in some vessels. These data provide the first direct evidence that elevated levels of in situ blood pressure induce K(Ca) channel expression in cerebrovascular smooth muscle membranes. This homeostatic mechanism may critically regulate the resting tone of cerebral arterioles during chronic hypertension. Furthermore, the overexpression of distinct K+ channel types during specific cardiovascular pathologies may provide for the upregulation of novel disease-specific membrane targets for vasodilator therapies.lld:pubmed
pubmed-article:9546382pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:languageenglld:pubmed
pubmed-article:9546382pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:citationSubsetIMlld:pubmed
pubmed-article:9546382pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9546382pubmed:statusMEDLINElld:pubmed
pubmed-article:9546382pubmed:monthAprlld:pubmed
pubmed-article:9546382pubmed:issn0009-7330lld:pubmed
pubmed-article:9546382pubmed:authorpubmed-author:LieIIlld:pubmed
pubmed-article:9546382pubmed:authorpubmed-author:RuschN JNJlld:pubmed
pubmed-article:9546382pubmed:authorpubmed-author:HudetzA GAGlld:pubmed
pubmed-article:9546382pubmed:authorpubmed-author:KnausH GHGlld:pubmed
pubmed-article:9546382pubmed:issnTypePrintlld:pubmed
pubmed-article:9546382pubmed:day6lld:pubmed
pubmed-article:9546382pubmed:volume82lld:pubmed
pubmed-article:9546382pubmed:ownerNLMlld:pubmed
pubmed-article:9546382pubmed:authorsCompleteYlld:pubmed
pubmed-article:9546382pubmed:pagination729-37lld:pubmed
pubmed-article:9546382pubmed:dateRevised2007-11-14lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:meshHeadingpubmed-meshheading:9546382-...lld:pubmed
pubmed-article:9546382pubmed:year1998lld:pubmed
pubmed-article:9546382pubmed:articleTitleIncreased expression of Ca2+-sensitive K+ channels in the cerebral microcirculation of genetically hypertensive rats: evidence for their protection against cerebral vasospasm.lld:pubmed
pubmed-article:9546382pubmed:affiliationDepartment of Physiology, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA.lld:pubmed
pubmed-article:9546382pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9546382pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:9546382pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:9546382pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9546382lld:pubmed