| pubmed-article:9528784 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C0178453 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:9528784 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:9528784 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9528784 | pubmed:dateCreated | 1998-5-21 | lld:pubmed |
| pubmed-article:9528784 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9528784 | pubmed:abstractText | The mei4+ gene of the fission yeast Schizosaccharomyces pombe was cloned by functional complementation. The mei4 disruptant failed to complete meiosis-I but could proliferate normally. mei4+ was transcribed only in meiosis-proficient diploid cells after premeiotic DNA replication. The mei4+ open reading frame encodes a 57-kDa serine-rich protein comprised of 517 amino acids with a forkhead/HNF3 DNA-binding domain in the amino-terminal region. Transcription of spo6+, a gene required for sporulation, was dependent on the mei4+ function. Two copies of the GTAAAYA consensus sequence, proposed as the binding site for human forkhead proteins, were found in the promoter region of spo6+. A gel mobility shift assay demonstrated the sequence-dependent binding of the GST-Mei4 forkhead domain fusion protein to DNA fragments with one of the consensus elements. Deletion of this consensus element from the spo6 promoter abolished the transcription of spo6+ and resulted in a sporulation deficiency. One-hybrid assay of Mei4 which was fused to the Gal4 DNA-binding domain localized the transcriptional activation domain in the C-terminal 140 amino acids of Mei4. These results indicate that Mei4 functions as a meiosis-specific transcription factor of S. pombe. | lld:pubmed |
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| pubmed-article:9528784 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9528784 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9528784 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9528784 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9528784 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9528784 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9528784 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9528784 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:TanakaKK | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:WatanabeYY | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:AbeHH | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:ShimodaCC | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:YamamotoMM | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:HorieSS | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:FujiokaHH | lld:pubmed |
| pubmed-article:9528784 | pubmed:author | pubmed-author:NishiwakiSS | lld:pubmed |
| pubmed-article:9528784 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9528784 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:9528784 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9528784 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9528784 | pubmed:pagination | 2118-29 | lld:pubmed |
| pubmed-article:9528784 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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