| pubmed-article:9520386 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9520386 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9520386 | lifeskim:mentions | umls-concept:C0023775 | lld:lifeskim |
| pubmed-article:9520386 | lifeskim:mentions | umls-concept:C0918012 | lld:lifeskim |
| pubmed-article:9520386 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:9520386 | pubmed:dateCreated | 1998-5-1 | lld:pubmed |
| pubmed-article:9520386 | pubmed:abstractText | Isoprostanes are prostaglandin isomers produced from arachidonic acid by a free radical-catalyzed mechanism. Urinary excretion of 8-iso-prostaglandin F2alpha, an isomer of the PGG/H synthase (cyclooxygenase or COX) enzyme product, prostaglandin F2alpha (PGF2alpha), has exhibited promise as an index of oxidant stress in vivo. We have developed a quantitative method to measure isoprostane F2alpha-I, (IPF2alpha-I) a class I isomer (8-iso-PGF2alpha is class IV), using gas chromatography/mass spectrometry. IPF2alpha-I is severalfold as abundant in human urine as 8-iso-PGF2alpha, with mean values of 737 +/- 20.6 pg/mg creatinine. Both isoprostanes are formed in a free radical-dependent manner in low density lipoprotein oxidized by copper in vitro. However, IPF2alpha-I, unlike 8-iso-PGF2alpha, is not formed in a COX-dependent manner by platelets activated by thrombin or collagen in vitro. Similarly, COX inhibition in vivo has no effect on IPF2alpha-I. Neither serum IPF2alpha-I, an index of cellular capacity to generate the isoprostane, nor urinary excretion of IPF2alpha-I, an index of actual generation in vivo, is depressed by aspirin or indomethacin. In contrast, both serum thromboxane B2 and urinary excretion of its 11-dehydro metabolite are depressed by the COX inhibitors. Although serum 8-iso-PGF2alpha formation is substantially depressed by COX inhibitors, urinary excretion of the compound is unaffected. Urinary IPF2alpha-I is elevated in cigarette smokers compared with controls (1525 +/- 180 versus 740 +/- 40 pg/mg creatinine; P < 0.01) and is highly correlated with urinary 8-iso-PGF2alpha (r = 0.9; P < 0.001). Urinary IPF2alpha-I is a novel index of lipid peroxidation in vivo, which can be measured with precision and sensitivity. It is an abundant F2-isoprostane formed in a free radical- but not COX-dependent manner. Although 8-iso-PGF2alpha may be formed as a minor product of COX, this pathway contributes trivially, if at all, to levels in urine. Urinary excretion of both isoprostanes is elevated in cigarette smokers. | lld:pubmed |
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| pubmed-article:9520386 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9520386 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9520386 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9520386 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9520386 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9520386 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9520386 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:FitzGeraldG... | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:LawsonJ AJA | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:RokachJJ | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:HwangS WSW | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:IulianoLL | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:PraticòDD | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:KhanapureS... | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:BarryO POP | lld:pubmed |
| pubmed-article:9520386 | pubmed:author | pubmed-author:AdiyamanMM | lld:pubmed |
| pubmed-article:9520386 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9520386 | pubmed:day | 31 | lld:pubmed |
| pubmed-article:9520386 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:9520386 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9520386 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9520386 | pubmed:pagination | 3449-54 | lld:pubmed |
| pubmed-article:9520386 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:meshHeading | pubmed-meshheading:9520386-... | lld:pubmed |
| pubmed-article:9520386 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9520386 | pubmed:articleTitle | IPF2alpha-I: an index of lipid peroxidation in humans. | lld:pubmed |
| pubmed-article:9520386 | pubmed:affiliation | Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia, PA 19104-6100, USA. | lld:pubmed |
| pubmed-article:9520386 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9520386 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9520386 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:9520386 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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