| pubmed-article:9518582 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0034085 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0000039 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0439962 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0001721 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C1442757 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:9518582 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:9518582 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:9518582 | pubmed:dateCreated | 1998-4-29 | lld:pubmed |
| pubmed-article:9518582 | pubmed:abstractText | Two methods were employed for preparation of lipid extracts from porcine lung surfactant. Pulmonary surfactant proteins SP-B and SP-C were isolated from the extracts using gel-exclusion chromatography on LH-60 with chloroform:methanol acidified with hydrochloric acid. Monolayers of pure SP-B or SP-C isolated from butanol lipid extracts spread at the air-water interface showed larger molecular areas than those determined in films of SP-B or SP-C isolated from chloroform surfactant extracts. Aqueous dispersions of dipalmitoylphosphatidylcholine (DPPC) supplemented with 2.5 and 5.0 wt% of SP-B or SP-C obtained from butanol extracts adsorbed faster to the air-water interface than their counterparts reconstituted with proteins isolated from chloroform extracts. Surface pressure-area characteristics of spread monolayers of DPPC plus SP-B or SP-C did not depend on the method of isolation of the proteins. The diagrams of the mean molecular areas vs. composition for the monolayers of DPPC plus SP-B or SP-C showed positive deviations from the additivity rule, independently of the procedure used for preparation of lipid extract surfactant. Matrix-assisted laser desorption/ionization spectrometry of the proteins isolated from different extraction solvents was consistent with some differences in the chemical compositions of SP-Bs. Butylation of SP-B during extraction of surfactant pellet with butanol may account for the differences observed in the molecular masses of SP-Bs isolated by the two different extraction protocols. The study suggests that the method of purification of SP-B and SP-C may modify their ability to enhance the adsorption rates of DPPC/protein mixtures, and this may be relevant to the formulation of protein-supplemented lipids for exogenous treatment of pulmonary surfactant insufficiency. | lld:pubmed |
| pubmed-article:9518582 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9518582 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9518582 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9518582 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9518582 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9518582 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:9518582 | pubmed:author | pubmed-author:StewartJJ | lld:pubmed |
| pubmed-article:9518582 | pubmed:author | pubmed-author:TaylorLL | lld:pubmed |
| pubmed-article:9518582 | pubmed:author | pubmed-author:KeoughK MKM | lld:pubmed |
| pubmed-article:9518582 | pubmed:author | pubmed-author:TanevaS GSG | lld:pubmed |
| pubmed-article:9518582 | pubmed:copyrightInfo | Copyright 1998 Elsevier Science B.V. | lld:pubmed |
| pubmed-article:9518582 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9518582 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:9518582 | pubmed:volume | 1370 | lld:pubmed |
| pubmed-article:9518582 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9518582 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9518582 | pubmed:pagination | 138-50 | lld:pubmed |
| pubmed-article:9518582 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:9518582 | pubmed:meshHeading | pubmed-meshheading:9518582-... | lld:pubmed |
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| pubmed-article:9518582 | pubmed:meshHeading | pubmed-meshheading:9518582-... | lld:pubmed |
| pubmed-article:9518582 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9518582 | pubmed:articleTitle | Method of purification affects some interfacial properties of pulmonary surfactant proteins B and C and their mixtures with dipalmitoylphosphatidylcholine. | lld:pubmed |
| pubmed-article:9518582 | pubmed:affiliation | Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, A1B 3X9, Canada. | lld:pubmed |
| pubmed-article:9518582 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9518582 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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